Abstract:

Abstract:Objective
To study the monoclones of SC189 and discuss the role of caspase-8 in glioblastoma resistance to tumor necrosis factor related apoptosis-inducing ligand(TRAIL)-induced apoptosis.Methods The monoclones were obtained from SC189,then the acid phosphate assay was used to detect the cell death rate to TRAIL,the expression of FADD,DR5,DR4 and caspase-8 in monoclones were detected by Western blotting,and then the caspases cleavage was checked after treated with TRAIL.Results The death percentage of cells of the monoclones isolated from SC189 was -5.25%-45.8% after treated with different doses of TRAIL,4 monoclones were resistant cell lines,only one monoclone was sensitive to TRAIL.Western blotting detection showed that the expressrons of FADD,DR5 and DR4 were similar.The expression of caspase-8  in resistant cell lines was decreased,and the cells hadn’t the cleavage reaction,otherwise,the expression of caspase-8 in sensitive cell lines was increased,and the cells had the caspases and DFF-45 cleavage after treated with TRAIL.Conclusion The expression of caspase-8 in SC189 monoclones is directly related to the sensitivity of TRAIL.

Key words: glioblastoma;tumor necrosis factor related apoptosis-inducing ligand;caspase-8