Abstract:

Abstract:Objective To investigate the effects of signal transducer and activator of transcription 3 (STAT3) on the invasiveness of pancreatic cancer cell line SW1990 and its mechanism.Methods  The SW1990 cells were divided into control group,negative group and siRNA group.The cells in siRNA group  were transfected with STAT3 siRNA,the expressions of STAT3 mRNA and protein were assayed by RT-PCR and Western blotting;the invasive ability of cancer cells was detected by Transwell assay.The expressions of MMP-2 and MMP-9 of pancreatic cancer cells were detected by RT-PCR and Western blotting.Results After transfected with STAT3 siRNA for 72h,the expressions of STAT3 mRNA and protein were significantly decreased,and there were significant differences compared with control group and negative group (P<0.05).The number of invaded cells in siRNA group was decreased significantly compared with  control group and negative group (P<0.05).At mRNA and protein levels,the expressions of MMP-2 and MMP-9 were significantly decreased in siRNA group,and there were significant differences compared with control group and negative group (P<0.05).Conclusion STAT3 siRNA could specifically block the activaty of STAT3 signal on SW1990 and  also inhibit the invasiveness of pancreatic cancer cells by down-regulating the MMP-2 and MMP-9 expressions.

Key words:  pancreatic neoplasms;signal trarsducer and activator of transcription 3;RNA interference;invasiveness