Abstract:

Abstract:Objective To study the effects of oviductus ranae on the immune system and prepare its microspheres.
Methods Fifty ICR mices were randomly divided into control group，model group，low dose oviductus  ranae group（0.1 g·kg^-1），middle dose oviductus ranae group（0.4 g·kg^-1）and high dose oviductus ranae group（1.0 g·kg^-1），the contents of spleen T lymphocyte subpopulations in mice were determined by flow cytometry，and the content of IL-2 in spleen T lymphocytes was detected by ELISA.The microspheres of oviductus ranae were prepared by spray drying, orthogonal experiment was used to investigate  the material ratio，inlet temperature，outlet temperature，feed flow rate with average particle size，range of particle size，drug loading and the degree of encapsulated efficiency，the optimum preparation of oviductus ranae microspheres was determined.Results Compared with  control group， the contents of spleen T lymphocyte subpopulations  CD3+，CD4+ levels and CD4+/CD8+ ratio in the mice in model group  were

significantly increased (P<0.001)；Compared with model group，the contents of spleen T lymphocyte subpopulations   CD3+，CD4+ levels in the mice in  low dose oviductus ranae group were decreased significantly（P<0.001），CD4+/CD8+ ratio was also decreased significantly（P<0.05）.Compared with  control group，the content of IL-2 in  spleen T lymphocytes   in the mice  in model group was decreased
significantly （P<0.001）；Compared with  model group，the contents of IL-2 in spleen T lymphocytes
in the mice in  low，middle and high dose  oviductus ranae groups   were significantly increased（P<0.001）；Spray drying was used to prepare microspheres，the optimum preparation was  A3B1C1D1，the material ratio was 1∶3，the inlet temperature was 130℃，the outlet temperature was 100℃，and the feed flow rate was 30 mL·min^-1.Conclusion Oviductus ranae has immune regulation,they can be  prepared into microspheres,  the technology is stable and suitable for industrial production.

Key words: Oviductus Ranae；immune regulation；microsphere；preparation technique