Abstract:

Abstract:Objective To explore the effects of velvet antler polypeptide/ nano β-tricalcium phosphate（β-TCP）/ gelatin material on the osteogenetic differentiation in rabbit mesenchymal stem cells (MSCs),and clarify the mechanism.Methods  Cell cycle and proliferation were examined by flow cytometry and MTT assay in the third generation MSCs treated with 5 mg/g velvet antler polypeptide/20 % nano TCP/gelatin material for 48 h.The growth of MSCs cocultured with the material was  observed by scanning electron microscope.The morphology  of apoptotic MSCs was observed by AO∕EB fluorescence staining assay.The activity of alkaline phosphatase (ALP) was detected with automatic biochemistry analyzer,and the mineralized nodules  in MSCs were counted after 21 d treatment.Results  The material could significantly promote the proliferation of MSCs,the relative growth rate was 126.45 % after treated with the material for 48 h.The percentage of MSCs in the S phase was 45.84 %（7.96% in control）,and the apoptotic  rate was 2.08 %（13.68%  in control group）.The calcified nodules and the activity of ALP were increased after treatment with material for 21 d. MSCs could adhere to the surface of the material and go well with it.Conclusion The material has the effects on stimulating the proliferation,differentiation and mineralization of rabbit MSCs.

Key words: nanostructures;mesenchymal stem cells;cell differentiation