Abstract:

Abstract:Objective To observe the effect of mongolian milkvetch root on nitric oxide(NO)，interleukin 5(IL-5)，inflammatory cells in bronchoalveolar lavage fluid (BALF) and serum of bronchial asthmatic rats and clarify the efficacy of mongolian milkvetch root in treatment of asthma．Methods 60 Wistar rats were randomly divided into normal group，model group，budesonide suspension for inhalation intervention group and low,middle,and high dose mongolian milkvetch root intervention groups，10 rats in each group．The rat bronchial asthma models were established by egg protein(OVA) sensibilization and long-term inhalation provocation．The rats in mongolian milkvetch root intervention groups were lavaged with mongolian milkvetch root 2 mL (0.3 g/100g)，and the rats  in  budesonide suspension for inhalation intervention group were  inhalated with  budesonide　suspension  2 mL at 21 d after expreriment;the rats in normal group and model group  were lavaged with normal saline orally，once each day for 14 d.The behavior of rats was observed and  the changes of NO，IL-5，inflammatory cells in BALF and serum of  bronchial asthmatic rats in each group were detected.Results The model of asthma was set up successfully by observing the behavior of rats，then the behavior of the rats became better after treatment，but did not reach the normal state.The NO  and IL-5 levels in  BALF and serum in mongolian milkvetch root intervention groups were significantly reduced compared with model group (P<0.01)，and the percentages of the total inflammatory cells and eosinophils in BALF were significantly reduced (P<0.01).Conclusion Mongolian milkvetch root could inhibit the increasing of  IL-5 and NO in BALF in asthmatic models，and could significantly reduce activation and infiltration of airway inflammatory cells and eosinophils，thus reduce airway inflammation and asthma symptoms or avoid the onset of asthma to achieve the purpose of prevention and treatment of asthma.

Key words: bronchial asthma;mongolian milkvetch root；nitric oxide； interleukin 5； inflammatory cells