Abstract:

Abstract:Objective To study the effects of ABM polysaccharide,flammulina velutipes polysaccharide and lentinan on the fermentation properties of lactobacillus acidophilus,and explore whether they can be considered as kinds of new prebiotics,and screen the best concentrations of the three fungi polysaccharides when they are used as prebiotics. Methods Fermented milk with lactobacillus acidophilus was studied by adding  different concerntrations of carbon sources. Experimental groups were added with ABM polysaccharide (0.2%,0.4%,0.6%,0.8%, and 1.0%）,flammulina velutipes polysaccharide (0.2%,0.4%,0.6%,0.8%, and 1.0%）and lentinan (0.4%,0.6%,0.8%,1.0%, and 1.2%）,positive control group was added with the traditional prebiotics inulin (1%,2%,3%,4%, and 5%）;the microbiological indexes,viscosities and pH values of  all  fermented milk samples were detected,and compared with negative control group.Results In the experiments of detecting microbial indicators,the viable count indexes were the largest when the ABM polyasccaride and flammulina velutipes polysaccharide concentrations were 0.6%,and the lentinan concentration was 0.8%,the results were 10.34,9.75 and 9.95 logcfu/mL;the viable counts of experimental groups were all larger than those in negative control group.In the experiments of detecting viscosity,the viscosity indexes were the largest when the ABM polyasccaride and lentinan concentrations were 0.8%,and flammulina velutipes polysaccharide concentration was 0.6%,the results were 3 800,3 300 and 2 800 mPa.s;the viscosities of experimental groups were all larger than those in  negative control group.In the experiments of detecting pH,the pH values were the lowest when the ABM polyasccaride and flammulina velutipes polysaccharide concentrations were 0.6%,and the lentinan concentration was 0.8%,the results were 4.74,5.12 and 5.08; the pH values of experimental groups were all lower than those in  negative control group.Conclusion ABM polysaccharide,flammulina velutipes polysaccharide and lentinan  have influences in the fermentation properties of lactobacillus acidophilus.All of them can reach the standard of being prebiotics.ABM polyasccaride is the best prebiotics in this study.

Key words: inulin;ABM polysaccaride;flammulina velutipes polysaccharide;lentinan;lactobacillus acidophilus