Abstract:

Objective
To investigate the effects of growth differentiation factor 5 (GDF5) on the proliferation and migration of endothelial cells,and clarify the mechanism of its repair. Methods Vascular endothelial cells were cultivated with different concentrations (10,50,100  μg?L-1) of GDF5, and normal control group was set up at the same time.The cell proliferation was detected by method of CCK8 method. Three-dimensional cultivation was used to investigate the effect of GDF5 on the migration of endothelial cells.Results CCK8 experiment showed that GDF5  inhibited the proliferation of vascular endothelial cells;with the increasing of  GDF5 dose,the inhibitory rate was increased correspondingly even more than twice,there were significant differences between various groups(P<0.05).Three-dimensional experiment showed that GDF5  obviously promoted the movement of vascular endothelial cells, with the increasing of   GDF5 dose,the cell movement was significantly increased,there were significant differences between GDF5 groups and control group(P<0.05).Conclusion GDF5 can inhibit the  proliferation of endothelial cells and promote the migration of endothelial cells.

Key words: growth differentiation factor 5;vascular endothelial cells;cell proliferation;cell migration