Abstract:

Objective
To investigate the influence of filamin A(FLNa) on drug-i
nduced apoptosis of breast cancer cells and explore its mechanism.Methods MDA-MB-231/MB 231-KD(FLNa+/ FLNa－) cells were treated with different doses(2.5,5.0,10.0 and 20.0 μmol?L-1)MMC,AS2O3 and DDP,meanwhile control group was set up.The inhibitory rates of proliferation of breast cancer cells were detected with MTT method.MDA-MB-231/MB231-KD(FLNa+/FLNa－)cells were treated with different doses (2.5，5.0，10.0 and 20.0 μmol?L-1)MMC,meanwhile control group was set up.The apoptotic rates of breast cancer cells were determined with flow cytometry.Results Compared with control group,the inhibitory rates of proliferation of FLNa+ and FLNa－ cells in  2.5,5.0,10.0,20.0 μmol?L-1 MMC groups were significantly increased(P<0.05),and were higher than those in AS2O3 and DDP groups(P<0.05)；there  was significant difference between  two kinds of breast cancer cells，the inhibitory rate of FLNa－ cells was higher than that of FLNa+ cells (P<0.05).As the dose increasing, the number of apoptotic cells was increased,there were significant differences compared with control group(P<0.05);the inhibitory rate of FLNa－ cells was higher than that of FLNa＋ cells and there was significant difference (P<0.05).Conclusion The expression of FLNa can reduce the  drug-induced apoptosis of breast cancer cells.

Key words: filamin A； apoptosis；breast neoplasms