Abstract:

Objective
To investigate the  inhibitory effect of proliferation of parthenolide(Par) on salivary gland adenoid cystic carcinoma cell line SACC-83 cells in vitro and clarify its related mechanism.Methods The SACC-83 cells were treated with different concentrations(5.0，7.5，15.0，30.0，40.0 and 80.0 mg?L-1) of Par in vitro,the proliferation inhibition of SACC-83 cells was detected by MTT colorimetry,the cell cycle distribution and apoptosis status were analyzed by flow cytometry,the expression of Livin protein was determined by Western blotting.Results The growth of SACC-83 cells in Par groups was inhibited effectively in concentration-　and time-dependent manner.The  apoptotic rates in  Par  groups were 14.7%±2.1%,30.5%±3.4% and 38.7%±2.7%, respectively, after treated with  7.5,15.0 and 30.0 mg?L-1 Par for 24 h, there were significant differences compared with  control group (3.2%±0.9%,all P<0.01).The expressions of Livin proteins in Par groups were significantly down-regulated,there were significant differences compared with control group(P<0.05);and there were significant differences between different doses of Par groups（P<0.01）.Conclusion Par could obviously inhibit the proliferation of SACC-83 cells.The expression of Livin  protein may be related to this effect.

Key words: parthenolide；salivary gland neoplasms；apoptosis；livin protein