Abstract:

Abstract:Objective
To investigate the best time of protective effect of puerarin on alcoholism rat hearts by  comparing the intervention  effect of puerarin on heart in rats with alcoholism at different administration time.Methods The male SD rats were randomly divided into control group,alcoholism group,and puerarin groups（treatment group，simultaneous administration group and prevention group).The contents of aspartate aminotransferase (AST),creatine phosphokinase (CPK),superoxide dismutase(SOD),malondialdehyde (MDA),and the activities of Ca²⁺-Mg²⁺-ATPase and  Na^+-K⁺-ATPase were detected by enzymic method and colorimetric method.Results Compared with alcoholism group,the content of SOD,and the activities of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in puerarin groups were increased,the contents of MDA,AST and CPK were reduced(P<0.05 or P<0.01).Compared among puerarin groups each other,the results were better for instantaneous administration group than the other two groups（P<0.05 or P<0.01）,besides the MDA in acute alcoholism group had no significant difference(P>0.05).Conclusion The puerarin has protective  effect on heart of rats with acute and chronic alcoholism,especially when it is used simultaneously with alcohol.

Key words: puerarin;acute alcoholism, chronic alcoholism;heart