Abstract:

Abstract:Objective To investigate the effects of recombinant plasmid pshuttle-Egr1-shTRAIL stable transfection in combination with X-ray irradiation on the TRAIL protein expression and the apoptosis in human SMMC7721 hepatoma cells. Methods The pshuttle-Egr1-shTRAIL packaged with liposome was stably transfected into SMMC7721 cells in vitro.The shTRAIL protein expressions were measured with ELISA assay,Annexin V-FITC kit was adopted to measure the apoptosis of pshuttle-Egr1-shTRAIL cells,and the changes in survival rate of SMMC7721 cells were measured　with cell cloning assay.Results The TRAIL protein expressions in pshuttle-Egr1-shTRAIL plus different doses of irradiation groups  were  significantly increased compared  with 0 Gy group（P< 0.001）.The percentage of apoptotic cells was significantly higher than that in 0 Gy group (P<0.05 or P<0.001),and the survival rate of SMMC7721 cells was decreased significantly (P<0.05 or P<0.001).Conclusion The pshuttle-Egr1-shTRAIL stable transfection in combination with irradiation can significantly induce the apoptosis of SMMC7721 tumor cells and inhibit the cell proliferation.

Key words: pshuttle-Egr1-shTRAIL;gene clone;human hepatoma SMMC7721;irradiation;gene therapy