Abstract:

Abstract:Objective
To observe the changes of Th1,Th2 and and Th3/Tr1 cytokines IFN-γ,IL-10 and TGF-β after treated with ionizing radiation,and explore the radiationship between cell balance and radiation dose.Methods After whole-body irradiation with various doses （low dose:0, 0.075,0.100,0.200Gy;high dose: 0,1.000,2.000,4.000,6.000 Gy） for 16 h,ELISA was used to detect the conternts of IFN-γ (Th1-type cytokine),and IL-10 (Th2 type cytokine),TGF-β1(Th3/Tr1 type cytokine) in spleen and thymus.Results Compared with sham irradiation control group,the IFN-γ and TGF-β levels in spleen cells were decreased after  low-dose radiation (0.075-0.200 Gy),but there was no statistically significant difference（P>0.05）;however the IL-10 secretion was significantly lower than that in sham irradiation group (P<0.01,P<0.05).After  high-dose radiation (2.000-6.000 Gy),the IFN-γ,IL-10,and TGF-β levels in spleen cells were higher than those in sham irradiation group (P<0.01,P<0.05). The IFN-γ and IL-10 after low- or high-dose radiation expressed more than sham irradiation group (P<0.01,P<0.05).But the TGF-β level was lower than that in sham irradiation group after radiation at low doses (P<0.01),and it was significantly higher than that in sham irradiation group after exposured to high-dose radiation (P<0.01).Conclusion High and low-dose radiation may influence the secretion of Th3/Tr1 type cells regulatory factor TGF-β of mouse immune organs and paly a key role in different immune response effects  induced by different doses of radiation.

Key words: ionizing radiation； T cells subsets；interferon-γ；interleukin-10；transforming growth factor-β