Abstract:

To investigate the expressions of Ets family transcription factor ER81 in testis and epididymis of busulfan-treated mice,and explore its  function on the proliferation and differentiation of spermatogonia.Methods Busulfan peritoneal injection (10 mg•kg-1) was performed and mouse testis and epididymis were collected on the 0th,3rd,5th,8th,10th,18th days after injection, respectively.The mRNA expression levels of ER81 in samples were analyzed by semi-quantitative RT-PCR.Results Compared with the 0th day,the expression of ER81 in testis was significantly decreased on  the  5th day (P<0.01) and then recovered gradually.In epididymis,compared with the 0th day,the expression of ER81 on the 8th day was significantly decreased(P<0.05).Conclusion ER81 may modulate the differentiation of spermatogonia.

Key words: Ets family transcription factor ER81;testis;epididymis;busulfan;mouse