Abstract:

To explore the influences of low level hydroquinone (HQ) in biological characteristics and the expression of poly(ADP-ribose) polymerase-1(PARP-1)gene in rat bone mesenchymal stem cells(BMSCs),and elucidate the toxic effect of low dose HQ on bone marrow.Methods HQ dissolved in PBS buffer at the concentrations of 2.5,5.0 and 10.0 μmol•L^-1 were respectively given to BMSCs,and the cells treated with PBS only were used as control group.The cell viability and proliferation were detected with CCK-8 assay kit,the apoptosis of BMSCs  was measured  by Annexin V/PI apoptosis assay kit,while the PARP-1 gene expression was determined by reverse transcription real-time RT-PCR.Results Compared with PBS control group,the ability of cell proliferation raised obviously (P<0.05);the apoptotic rates of BMSCs in 5.0 and 10.0 μmol•L^-1  HQ groups 48 h after exposure were significantly decreased (P<0.05).The expressions of PARP-1  in 2.5,5.0,and 10.0 μmol•L^-1　HQ groups 48　h after exposure were 0.92,0.56(P<0.05),and 0.45 folds of control group(P<0.05).Conclusion Low level HQ could inhibit the apoptosis of BMSCs and PARP-1 gene expression,while promote the cell growth.

Key words:  hydroquinone;bone mesenchymal stem cells;poly（ADP-ribose）polymerase-1;apoptosis;cell proliferation;rats