Abstract:

Abstract:Objective To investigate the effect of lancemaside D on the time effects of cell proliferation and cell cycle of HepG-2 cells and explore the anticancer active ingredients and mechanisms of codonopsis lanceolata. Methods The HepG-2 cells were treated with different concentrations (5,10,15,20 and 25 mg/L) of lancemaside D in vitro,meanwhile control group was set up. The proliferation inhibition of HepG-2 cells was detected by MTT assay,and the change of cell cycle  of HepG-2 cells was determined by flow cytometry. Results The results showed that the  inhibitory rate of cell proliferation of HepG-2 cells were  significantly higher than that in control group(P<0.01) after treated with 25 mg/Llancemaside D for 12,24,48 and 72 h;the cell numbers in lancemaside D groups were significantly smaller than that in control group after treated with lancemaside D for 48,72,96 and 120 h (P<0.05),among 5,15,20 and 25 mg/Lgroups there were significant differences (all P<0.05);the HepG-2 cell proliferation speed was delated with the increasing of lancemaside D concentration. The cell cycle detection results showed that in the lancemaside D concentration range of 10-25 mg/L,the percentages of HepG-2 cells at  G0/G1 phase were 55.97%±0.42%,57.16%±0.13%,57.48%±0.15% and 60.39%±0.32%, respectively,they were significantly higher than that in control group(53.22%±0.28%)(P<0.05). In the same concentration range of lancemaside D,the percentages of HepG-2 cells at S phage were 27.47%±1.42%,26.15%±2.71%,26.34%±0.67% and 24.81%±0.46%,they were significantly lower than that in control group(33.47%±0.25%)(P<0.05),which suggested a G0/G1 cell cycle arresting in lancemaside D group. Conclusion Lancemaside D strongly inhibits the growth of HepG-2 cells in vitro and furthermore cause cell DNA metabolism disturbance.

Key words: lancemaside D;HepG-2;growth curve;cell proliferation;cell cycle