Abstract:

Objective To observe the effects of 4,5,6,7-tetrabromobenzotriazole(TBB) on the expressions of CK2β and Akt in thyroid squamous cell carcinoma cell line (SW579) and to explore their mechanisms.Methods SW579 cells were cultured in vitro and divided into con
trol group,DMSO group，TBB (25 μmol/L) group， and Wortmannin (100 nmol/l group randomly.The inhibitory rate of the growth of SW579 cells was detected with MTT and the protein kinase activity was measured.The CK2β and Akt mRNA and protein expression levels were determined by RT-PCR and Western blotting.Results Compared with control group,the inhibitory rate of growth of SW579 in TBB group was increased（P<0.01） and the cellular activity of CK2 was decreased（P<0.01）. Compared with control group,the expression levels of CK2β mRNA and protein  in TBB group were significantly reduced（P<0.01）,while Akt mRNA and protein levels were unchanged（P>0.05）,but phosphorylation status of Thr308 and Ser473 of Akt  in TBB group was inhibited（P<0.01）.Conclusion TBB can inhibit the expression and activity of CK2β and down-regulate the activity of Akt of SW579 cells.

Key words: 4,5,6,7-tetrabromobenzotriazole；thyroid squamous cell carcinoma；CK2β；Akt/PKB； SW579