Abstract:

Objective
 To study the effect of different cultivate time,ultrasonic time and ultrasonic power on the concentration,type and quantity of the  intracellular protein from Candida albicans and Fusarium solina and to provide the theoretical basis for establishing a reliable and stable extraction method for intracellular protein from fungus.Methods Candida albicans and Fusarium solina were cultured.The different parameters included the culture time(36,48,and 72 h),ultrasonic time(20 s,3 min,5 min,and 10 min) and ultrasonic power(332.5 W and 475.0 W).The concentrations,species and quantities of protein under different factors were detected by the protein concentration detection and SDS-PAGE electrophoresis analysis.Results Both strains got the most quantity of protein when cultivated for 36 h.The most quantity and species of Candida albicans were obtained when the ultrasonic power was 332.5 W and ultrasonic time was 3 min,but as to Fusarium solina,the corresponding parameters were 332.5 W and 10 min, respectively.Conclusion The intracellular protein is extracted by compositely using ultrasonic method and glass bead transformation method.The optimal extraction method of intracellular protein from fungus is developed from the aspects of culture time,ultrasonic power and ultrasonic working time of strains.

Key words: Candida albicans;Fusarium solani;ultrasound;culture time