Abstract:

Objective
To isolate  the flavonoid glycosides from Scorzonera austriaca Willd and identify its structure and to provide experimental evidences for medicinal values of flavonoid glycosides from Scorzonera austriaca Willd. Methods The herbs of Scorzonera austriaca Willd was extracted with 70% ethanol,the extractives were purified with macroporous resin,silica gel,ODS column chromatography and HPLC and the structure of componuds were identified. Results Three compounds were isolated,and their structures were identifited through spectroscopic analysis and physico-chemical constants.They were 5,7,4′-trihydroxy-flavone-8-C-β-D-glucopyranoside,5,7,4′-trihydroxy-flavone-6-C-α-L-arabinosyl-8-C-β-D-glucopyranoside and 5,7,3′,4′-tetrahydroxy-flavone-6-C-α-L-arabinosyl-8-C-β-D-glucopyranoside.Conclusion Three compounds are all regarded as flavonoid lycosides isolated from Scorzonera austriaca Willd for the first time.
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Key words: Scorzonera austriaca Willd;flavonoid glycoside;extraction and isolation;structure identification