Abstract:

Objective
To develop the method of high performance liquid chromatography(HPLC) for determination of curcumin and curcumin derivative A in rat plasma and study their  pharmacokinetics in rats, and to provide basis for  clinical application of curcumin.Methods The curcumin and the curcumin derivative A were lavaged to the rats,and the concentrations of curcumin and curcumin derivative A in rat blood at different time points were determined by HPLC.The curcumin and curcumin derivative A were separated well on a Hypersil ODS2 C18 （5  μm×4.6 mm　×200 mm）under 30℃.The mobile phase of curcumin was a mixture of acetonitrile-water containing 1% acetic acid (45∶55) at a flow rate of 1 mL?min-1.The detective wavelength was 420 nm;the mobile phase of curcumin derivative A was a mixture of acetonitrile-water containing 1% acetic acid (70∶30) at a flow rate of 1 mL?min-1.The detective wavelength was 361 nm.Results The calibration curve was liner at the range of 0.25-100.00 mg?mL-1.The regression equation of curcumin was  Y=105.90X－22.70，r=0.999 6；the regression equation of curcumin derivative A was Y=71.20X+24.62，r=0.999 4.The RSD of intra-day precision and inter-day precision was less than 4%.The average recovery was more than 96%.The plasma concentration-time curves of curcumin and curcumin derivative A in rats  fit to one compartment model respectively.The clearance rate,the area under temperature and t1/2 of curcumin derivative A were 3.57,4.09 and 2.79 times respectively than those of curcumin.Conclusion The method of HPLC for the determination of curcumin and curcumin derivative A in plasma is accurate,simple and reliable,which provides the basis for the improvement of stablility and the extending of the half-time of curcumin.

Key words: curcumin；curcumin derivative；pharmacokinetics；high performance liquid chromatography