Abstract:

Abstract:Objective
To study the long-term effects of lasting and high-dose  electromagnetic radiation on number and percent of peripheral immune cells in mice and explore the influence of the lasting and high-dose electromagnetic radiation in murine immune system.Methods  Thirty BALB/c mice were randomly divided into control group,CTX group and irradiation group.The mice in irradiation group were exposed to electromagnetic radiation at the power density of 10 mW•cm^-2,30 min a day,for 5 consecutive days every week,lasting for 4 weeks.The mice in CTX group were fed with CTX with the concentration of 30　mg•kg^-1,for seven times.The number and percents of CD4+T cells,CD8+T cells,CD4+/CD8+,CD49+ NK cells in peripheral blood cells were detected 30,45,60,75,90,105 and 120 d after electromagnetic radiation.Results After high-dose electromagnetic radiation,the number of WBC was increased obviously (P<0.05).The number of CD4+ T cells was increased on the 75th day (P<0.05) and began to decrease on the 90th day,while the number was much more than that in control group (0 mW•cm^-2).Until the 120th day,the number of T cells was as the same as the control.After the long-term effect of lasting and high-dose electromagnetic radiation,the number of CD8+T cells was decreased,and from the 90th day to the 120th day the number　of T cells became normal (P<0.05).The ratio of CD4+/CD8+ and the number of CD49+ NK cells were lower than those in control group after irradiation (P<0.05).
Conclusion Chronic,persistent large doses of electromagnetic radiation can lead to immune system disorders,through detecting the  changes in the number of peripheral blood CD4+ T cells,CD8+ T cells,CD49+ NK cells and the proportion of CD4+/CD8+,the immune status of mice after irradiation can be found.

Key words: electromagnetic radiation;immune cells;mice,inbred BALBC