Abstract:

Abstract:Objective  To analyze the influence of cyclooxygenase-2(COX-2) inhibitor NS-398 on cell morphology,cell cycle,proliferation and apoptosis of hepatocelluar carcinoma cells SMMC-7721,and to investigate the mechanism of the effect of NS-398   on the growth of tumor cells. Methods The SMMC-7721 cells were treated with different doses of NS-398 (0,25,50,75,100,and 150 μmol/L) in various groups  for 24,48 and 72 h,and at the same time normal control group was set up.The changes of cell morphology in various groups were observed,the inhibitory rates  of proliferation  of hepatocellular carcinoma cells were detected by MTT assay,and the changes of cell cycle and apoptotic rates  were detected  by flow cytometry. Results After treated with 25,50,75,100,and 150 μmol?L-1 NS-398,the cell adhesion was decreased,and some of the cells were floating,and the medium was not clear.Compared with normal control group,the ratios of SMMC-7721 cells in  G0/G1 phase were reduced,and the ratios of SMMC-7721 cells in  G2/M phase were increased in the other groups.No apoptotic peak was observed in normal control group.The apoptotic peak was observed in 50 μmol?L-1 NS-398 group after treated for 24 h,and  the level of apoptosis was increased significantly  72 h after treatment.The  inhibitory rates of proliferation   had a concentration- and time-dependant trend in various groups.Conclusion The COX-2 inhibitor NS-398 can repress the proliferation  of  hepatocellular carcinoma   cells SMMC-7721 and induce   apoptosis;the process is time- and dose-dependant.

Key words: 环氧合酶-2, 抑制剂, 肝细胞肿瘤, 流式细胞术