Abstract:

Objective To explore the therapeutic effect of phenylethanoid glycosides on cyclophosphamide (CTX)-induced dyszoospermia in mice and to preliminary elucidate the mechanisms involved in the process. Methods Phenylethanoid glycoside was extracted by ethanol extraction. Forty male BALB/C mice were randomly divided into control group,model group,low dose of  phenylethanoid glycosides group (50 mg/kg^-1) and high dose of phenylethanoid glycosides group (100 mg/kg^-1). Except control group,the dyszoospermia mouse model was established by peritoneal injection of CTX at the daily dose of 80 mg?kg-1,once daily for  successive 5 d. After modeling,phenylethanoid glycosides were intragastrically administered at corresponding doses to each phenylethanoid glycosides group. Equal volume of
 normal saline was given to the mice in control group and model group by gastrogavage. All the medication was performed once daily for successive 30  d. The testis tissue was  obtained 24 h after the last intragastric administration. The level of testosterone in the testis tissue homogenate was determined by enzyme linked immunosorbent assay. The sperm counts,the motility rates,and the teratospermia rates in various groups were compared. The morphological changes of the testis tissue were observed using HE staining. Results Compared with control group,the sperm count and the motility rate were decreased,the teratospermia rate was increased,and the testosterone level in the testis tissue homogenate was decreased in model group(P<0.01). Compared with model group,the sperm counts and the motility rates were increased,the teratospermia rates were decreased,and the testosterore  levels in the testis tissue homogenate were increased in phenylethanoid glycosides groups (P<0.05 or P<0.01). The histological results showed atrophy and degeneration of seminiferous tuble, thicker seminiferous epithelium  and azoospermic lumina in  model group; the number of seminiferous epithelial layers was increased and the seminiferous cells orderly arranged,and many sperms were found in the tubules in  phenylethanoid glycosides groups. Conclusion Phenylethanoid glycosides has obviously therapeutical effect on CTX-induced dyszoospermia in mice,and its mechanisms might be correlated with recovering the testosterone level.
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Key words: phenylethanoid glycosides;cyclophosphamide;spermatogenic impairment, mice,BALB/C
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