Journal of Jilin University Medicine Edition ›› 2015, Vol. 41 ›› Issue (02): 295-298.doi: 10.13481/j.1671-587x.20150217

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Effects of dibutyl phthalate on expression of Cx43 protein and testosterone excretion in Leydig cells of testicle in adult rats

JIN Shuguang, ZHANG Yi, LI Lingyi, LI Huan   

  1. Department of Environmental Hygiene, Institute of Public Health, Beihua University, Jilin 132013, China
  • Received:2014-09-16 Online:2015-03-28 Published:2015-04-04

Abstract:

Objective To explore the effect of dibutyl phthalate(DBP) on the expression of Cx43 protein and testosterone excretion in the Leydig cells of testicle in the adult rats,and to disscuss whether there is relationship between the regulation of testosterone by DBP and Cx43.Methods After the adult SD rats were killed, the cultured primary pure Leydig cells were divided into solvent control group(0.1%DMSO),DBP group (50 mg·L-1 DBP),Cx43 enhancer group(50 mg·L-1 DBP+10 μmol·L-1 PGE2),and specific testosterone blocker group (40 mg·L-1 flutamid).After cultured for 24 h,radioimmunoassay was used to measure the levels of testosterone in the Leyding cells; cell immunofluorescence and Western blotting method were performed to detect the expressions of Cx43 protein in the Leydig cells.Results Compared with DMSO group,the level of testosterone and the expression level of Cx43 protein in the Leydig cells in DBP group were decreased after 24 h exposure(P<0.05).Compared with DBP group,the testosterone level in DBP+PGE2 group had no obvious change(P>0.05),but the expression level of Cx43 protein was increased(P<0.05).Compared with DMSO group,the level of testosterone and the expression level of Cx43 protein in flutamide group were decreased significantly(P<0.05).Conclusion DBP has the effects on the Cx43 expression and testosterone excreation,and Cx43 has no inhibitory effect on the synthesis of testosterone.However,testosterone can regulate the expression of Cx43 reversely.Therefore,it's not necessary to take Cx43 as the target of DBP in adjusting the synthesis of testosterone in Leydig cells.

Key words: dibutyl phthalate, Leydig cells, Cx43, testosterone

CLC Number: 

  • R961