PDGF/ROCK通路在AcSDKP抑制大鼠矽肺纤维化形成中的作用

doi:10.13481/j.1671-587x.20150312

Abstract

Abstract:

Objective To investigate whether N-acetyl-seryl-aspartyl-lysyl-proline (AcSDKP) can inhibit the development of silicosis through blocking PDGF/ROCK pathway. Methods SiO₂ were douched in bronchial tube of the rats to make the silicotic models.Sixty Wistar rats were randomly divided into model control 4 weeks group, model control 8 weeks group, silicosic model 4 weeks group, silicosic model 8 weeks group, AcSDKP post-treatment group and AcSDKP pre-treatment group (n=10).The expression and distribution of phospho-PDGFR-β in lung tissue of the rats in various groups were observed by immunocytochemistry.The expressions of α-smooth muscle actin (α-SMA), PDGFR-β, phospho-PDGFR-β, ROCK, Ⅰ collagen and Ⅲ collagen in lung tissue of the rats in various groups were detected by Western blotting method. Results Compared with the corresponding control groups, the expression levels of α-SMA, PDGFR-β, phospho-PDGFR-β, ROCK, type Ⅰcollagen and type Ⅲ collagen in lung tissue of the rats in silicosic model groups were increased significantly(P<0.05).In addition, in silicosic model groups, there were more phospho-PDGFR-β positive cells distributed in the silicon nodules and interstitial fibrosis area detected by immunocytochemistry.However, these effects were inhibited in AcSDKP post-treatment group and AcSDKP pre-treatment group (P<0.05).The Results of immunohistochemical staining showed the expression of phospho-PDGFR-β protein was obviously decreased in AcSDKP post-treatment group and AcSDKP pre-treatment group. Conclusion PDGF/ROCK pathway may play an important role in the development of silicosis by promoting myofibroblast differentiation and further secreting more collgen; however, these effects may be inhibited by AcSDKP.

Key words: N-acetyl-seryl-aspartyl-lysyl-proline, platelet-derived growth factor, Rho-associated coiled-coil-forming protien kinase, α-smooth muscle actin, myofibroblast, silicosis

CLC Number:

R135.2

ZHANG Lijuan, LI Qian, XU Hong, LI Shuyu, PEI Xin, ZHANG Wenli, YANG Fang. Effect of PDGF-ROCK pathway in process of N-acetyl-seryl- aspartyl-lysyl-proline inhibiting development of pulmonary fibrosis in rats with silicosis[J].Journal of Jilin University Medicine Edition, 2015, 41(03): 496-500.

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Effect of PDGF-ROCK pathway in process of N-acetyl-seryl- aspartyl-lysyl-proline inhibiting development of pulmonary fibrosis in rats with silicosis

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