MMP-2和MMP-9在食管鳞状细胞癌患者癌组织中的表达及其意义

doi:10.13481/j.1671-587x.20150428

Abstract

Abstract:

Objective To explore the expressions of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) in the tumor tissue of the patients with esophageal squamous cell carcinoma, and to analyze the correlation between the expressions of MMP-2 and MMP-9. Methods 100 spicemens of surgical removed esophageal squamous cell carcinoma were chosen. The tumor tissue and the adjacent normal esophagel mucosa tissue were selected.RT-PCR method was used to detect the expressions of MMP-2 and MMP-9 mRNA in the esophageal squamous cell carcinoma tissue and the adjacent normal tissue.Western blotting method was used to testify the expressions of MMP-2 and MMP-9 proteins;the correlation between the expressions of MMP-2 and MMP-9 was analyzed by Pearson correlation analysis. Results The positive expression rate of MMP-2 mRNA in carcinoma tissue was 89.0%(89/100), the positive expression rate of MMP-2 mRNA in adjacent normal tissue was 76.0%(76/100),and there was statistical difference between two groups(χ²=5.853,P=0.016).The positive expression rate of MMP-9 mRNA in carcinoma tissue was 48.0%(48/100),the positive expression rate in adjacent normal tissue was 16.0%(16/100),and there was statistical difference of the positive expression rate between two groups(χ²=23.529,P=0.000).There was a positive correlation between the expression of MMP-2 mRNA and the tumor invasion depth(χ²=4.971,P=0.026), but there was no correlation between the expression of MMP-2 mRNA and the lymph node metastasis(χ²=2.127,P=0.145).There was a positive correlation between the expression of MMP-9 mRNA and the tumor invasion depth(χ²=4.421,P=0.035), but there was no correlation between the expression of MMP-9 mRNA and lymph node metastasis(χ²=0.617,P=0.432). There was positive correlationship between the positive expression rates of MMP-2 mRNA and MMP-9 mRNA in esophageal cancer tissue(r=0.274,P=0.006). Conclusion The MMP-2 and MMP-9 may play a very important role in the occurrence and development of esophageal squamous cell carcinoma,however they have no affection on lymph node metastasis of esophageal squamous cell carcinoma.

Key words: esophageal neoplasms, carcinoma,squamous cell, matrix metalloproteinase-2, matrix metalloproteinase-9, reverse transcription-polymerase chain reaction

CLC Number:

R735.1

SUN Xiaohong, FANG Fuyuan, LI Hui, YIN Na, PANG Zuoliang, LI Huiwu. Expressions of MMP-2 and MMP-9 in tumor tissue of patients with esophageal squamous cell carcinoma and their significances[J].Journal of Jilin University Medicine Edition, 2015, 41(04): 814-819.

References

[1] Jemal A, Bray F, Center MM, et al.Global cancer statistics [J].CA Cancer J Clin,2011,61(2):69-90.

[2] 张思维,张敏,李光琳,等.2003-2007年中国食管癌发病与死亡分析[J].中国肿瘤,2012,21(4):241-247.

[3] Wei WQ,Yang J,Zhang SW,et al.Analysis of the esophageal cancer mortality in 2004-2005 and its trends during last 30 years in China[J].Zhonghua Yu Fang Yi Xue Za Zhi,2010,44(5):398-402.

[4] 李秀梅,陈艳,王洪江,等.哈萨克族食管癌中MMP-1、MMP-2、MMP-7、TIMP-1和MTA1的表达及其临床病理意义[J].癌变·畸变·突变,2011,23(3): 190-193,198.

[5] 刘敏,郭晓娟,张红霞,等.食管癌中EGFRvⅢ、MMP-2的表达特点及意义[J].现代肿瘤医学,2010,18(1): 62-66.

[6] 陈超伍,马洪升.基质金属蛋白酶在食管癌组织中的表达及与微量元素含量之间的关系[J].世界华人消化杂志,2010,18(19):1995-2000.

[7] 岳庆峰,向明,李方明,等.食管癌组织DcR3、MMP-2表达及其对生存率的影响[J].中国癌症杂志,2010,20(10):745-750.

[8] Li Y,Ma J,Guo Q,et al.Overexpression of MMP-2 and MMP-9 in esophageal squamous cell carcinoma[J].Dis Esophagus,2009,22(8):664-667.

[9] Hong SW,Kang YK,Lee B,et al.Matrix metalloproteinase-2 and -7 expression in colorectal cancer[J].Korean Soc Coloproctol,2011,27(3):133-139.

[10] Koskensalo S,Hagström J,Linder N,et al.Lack of MMP-9 expression is a marker for poor prognosis in Dukes' B colorectal cancer[J].BMC Clin Pathol,2012,12(7):12-24.

[11] Hwang TL,Lee LY,Wang CC,et al.Claudin-4 expression is associated with tumor invasion,MMP-2 and MMP-9 expression in gastric cancer[J].Exp Ther Med,2010,1(5):789-797.

[12] 于维娜.MMP-2、TIMP-2、PINCH在食管鳞状细胞癌中的表达意义及相关性研究[D].石家庄:河北医科大学,2011.

[13] 董刚强,王永占,蒲红,等.基质金属蛋白酶MMP-9在胃癌中的表达及其临床意义 [J].肿瘤预防与治疗,2012,25(4):240-242.

[14] 陈其军,吕自力,党裔武,等.MMP-9和TIMP-1在胃癌组织中的表达失衡及其与ZHX2的相关性 [J].世界华人消化杂志,2012,20(20):1832-1837.

[15] Chen Y,Jiang T,Mao A,et al.Esophageal cancer stem cells express PLGF to increase cancer invasion through MMP9 activation [J].Tumour Biol,2014,35(12):12749-12755.

[16] Li Y,Guo H,Dong D,et al.Expression and prognostic relevance of cyclophilin A and matrix metalloproteinase 9 in esophageal squamous cell carcinoma [J].Diagn Pathol,2014,18(8): 207-212.

[17] 关耀武.食管鳞状细胞癌组织中VEGF、MMP-9的表达及意义[J].山东医药,2011,51(6):86-87.

[18] Durlik M,Gardian K.Metalloproteinase 2 and 9 activity in the development of pancreatic cancer [J].Pol Przegl Chir,2012,84(8):377-382.

[19] Oshima T,Kunisaki C,Yoshihara K,et al.Clinicopathological significance of the gene expression of matrix metalloproteinases and reversion-inducing cysteine-rich protein with Kazal motifs in patients with colorectal cancer:MMP-2 gene expression is a useful predictor of liver metastasis from colorectal cancer [J].Oncol Rep,2008,19(5):1285-1291.

[20] Lu CL,Ji Y,Ge D,et al.The expression of CXCR4 and its relationship with matrix metalloproteinase-9/vascular endothelial growth factor in esophageal squamous cell cancer [J].Dis Esophagus,2011,24(4):283-290.

Expressions of MMP-2 and MMP-9 in tumor tissue of patients with esophageal squamous cell carcinoma and their significances

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0

[1]	XU Jiguang, LI Yuning. Therapeutic effect of folic acid combined with vitamin B12 on atherosclerosis induced by hyperhomocysteinemia in rats [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 943-947.
[2]	YANG Jie, MA Yinghui, CUI Xiucheng, WANG Hongmei, DONG Weili, TAN Xiaobo. Expression of MMP-2 in retina tissue of diabetic retinopathy model rats [J]. Journal of Jilin University Medicine Edition, 2017, 43(05): 923-927.
[3]	ZHANG Ning, WANG Jianfeng, CHENG Guanghui, HE Mingyuan. Evaluation on curative effect of Xihuang Capsule in patients with advanced esophageal cancer treated with chemoradiotherapy [J]. Journal of Jilin University Medicine Edition, 2017, 43(04): 812-817.
[4]	WANG Qin, DU Liqing, WANG Yan, XU Chang, LI Jin, LIU Qiang. Inhibitory effect of Rb94 gene combined with radiotherapy on growth of esophageal carcinoma cells of tumor-bearing nude mice [J]. Journal of Jilin University Medicine Edition, 2017, 43(02): 220-224.
[5]	YAO Jindan, HAN Guanghong, HU Min. Effects of IL-17 on expressions of MMP-9 in osteoclasts and their significances [J]. Journal of Jilin University Medicine Edition, 2016, 42(03): 462-466.
[6]	KOU Wei,DOU Chun-jiang,ZHOU Yun-song,LAN Yong-mei,GU Qiao-ling. Expressions of MTSS1 and Cap43 in esophageal squamous cell carcinoma tissue and their clinical significances [J]. Journal of Jilin University Medicine Edition, 2014, 40(04): 842-846.
[7]	ZHANG Lu-chuan,WANG Feng,FAN Qing-xia . Expressions of TFPI-2 and MMP-9 in esophageal squamous cell carcinoma tissue and their relationship with vasculogenic mimicry  [J]. Journal of Jilin University Medicine Edition, 2014, 40(03): 664-667.
[8]	LIU Bao-ling,FU Tong,LIU Bing,SUN Min-dan . Influence of tacrolimus on expression levels of MMP-9 and TIMP-1 in kidney tissue of rats with IgA nephropathy [J]. Journal of Jilin University Medicine Edition, 2014, 40(02): 289-293.
[9]	SUN Xiao-hong,LYU Hong-bo,LI Hui,YIN Na,LI Hui-wu,PANG Zuo-liang. Expressions of MMP-9 and TIMP-1 mRNA in esophageal  squamous cell carcinoma tissue and their significances [J]. Journal of Jilin University Medicine Edition, 2013, 39(6): 1247-1251.
[10]	WANG Xiu-lan,ZHAO Tong,CHEN Dan-feng,LI Lei,GUO Hai-yan,CHI Bao-rong. Relationship between p18 mRNA expression and esophageal cancer [J]. J4, 2011, 37(5): 919-922.
[11]	ZHANG Zhao-zhi,MENG Xiao-ping,WANG Li. Detection of serum TNF-α\|IFN-γ\| MMP-9 and oxLDL levels in patients with acute coronary syndrome and its clinical significances [J]. J4, 2011, 37(3): 534-537.
[12]	SUN Xun, MENG Xiang-Wei, ZOU YA-Bin, LIU Yan, WANG Xin-Hong. Application of immunohistochemical staining in diagnosis of esophageal spindle cell tumor [J]. J4, 2010, 36(5): 962-964.
[13]	ZHU Dan, SHENG Min-Jia, FU Jiu-Yuan, SU Hai-Lan. Expressions of matrix metalloproteinase-2 and matrixmetalloproteinase-9 in endometrioid adenocarcinoma tissues and clinical significances [J]. J4, 2010, 36(5): 965-968.
[14]	HE Ming-rui, DONG Yu, FU Tong, SUN Min-Dan. Effects of Zn on \|kidney of dibetic rats and expessions of VEGF,MT and MMP-9 in kidney tissues [J]. J4, 2010, 36(4): 656-659.
[15]	LI Hui-Min, LI Yi-Lei, SONG Xue-Fei, DONG Xin-Ji, LIU Zhi-Jing, LIANG Shan-Shan, ZHANG Li-Hong, LI Wei. Expressions of MMP-26,MMP-9 and TIMP-4 in cervical carcinoma tissues and their significances [J]. J4, 2010, 36(2): 354-358.