S100钙结合蛋白P在胃癌患者胃癌组织和血清中的表达及其临床意义

doi:10.13481/j.1671-587x.20150431

Abstract

Abstract:

Objective To investigate the relationship between the expressions of S100 calcium-binding protein P (S100P) in gastric cancer tissue and serum of the patients with gastric cancer and their clinicopathological parameters and monitor the serum S100P protein levels before and after tumor resection,and to explore its correlation with the prognosis of gastric cancer. Methods 50 patients with gastric cancer confirmed by surgical resection and pathological examination were selected.The pre-operative serum and post-operative gastric cancer tissue paraffin samples were collected.The fresh tissue samples and adjacent tissue were adopted from 20 patients with gastric cancer,and the postoperative dynamic serum samples were obtained from the other 30 patients with gastric cancer.Meanwhile,30 health volunteers were selected as normal control group.The expression level of S100P mRNA in gastric cancer tissue was detected by semi-quantitative RT-PCR.Immunohistochemistry and enzyme linked immunosorbent assay (ELISA) were used to detect the expression levels of S100P protein both in gastric cancer tissue and serum. Results The expression level of S100P mRNA in gastric cancer tissue was significantly lower than that in adjacent normal tissue (P<0.05);the positive expression rate of S100P protein in gastric cancer tissue was significantly lower than that in adjacent tissue (P<0.05);the down-regulation of the expression of S100P protein in gastric cancer tissue was positively correlated with vascular cancer embolus (P<0.05).The serum S100P level in the patients with gastric cancer before operation was lower than that in normal control group,but there was no significant difference (P> 0.05).The serum S100P level in the gastric cancer patients without lymph node metastasis was higher than that in the patients with lymph node metastasis,but there was no significant difference (P> 0.05).The serum S100P level in the patients with gastric cancer 2 weeks after operation was significantly lower than before operation. Conclusion The expression levels of S100P in gastric cancer tissue and serum of the patients with gastric cancer are decreased,and its expression level is correlated with the prognosis. The continuous dynamic monitoring of serum S100P level may predict the recurrence and prognosis.

Key words: S100 calcium-binding protein P, stomachneoplasms, prognosis

CLC Number:

R735.2

QIAO Xiaojuan, SU Xiulan, SHI Yonghong, DU Hua, ZHANG Shucai, LI Yunxia. Expressions of S100 calcium-binding protein P in gastric cancer tissue and serum of patients with gastric cancer and their clinical significances[J].Journal of Jilin University Medicine Edition, 2015, 41(04): 830-835.

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Expressions of S100 calcium-binding protein P in gastric cancer tissue and serum of patients with gastric cancer and their clinical significances

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