二甲双胍对糖尿病小鼠肝库普弗细胞炎症反应的抑制作用和吞噬功能的改善作用

doi:10.13481/j.1671-587x.20190108

Abstract

Abstract: Objective: To observe the inhibitory effect of metformin on the inflammatory response of Kupffer cells(KCs) of liver in the diabetic mice, and to explore its improvement on the phagocytic function of KCs, and to elucidate the protective mechanism of metformin on the KCs in the diabetic mice.Methods: The C57BLKS/J db/db mice were divided into diabetes group and diabetes + metformin group, and the C57BLKS/J db/m mice were divided into non-diabetes group and non-diabetes + metformin group, 8 mice in each group. The KCs were cultured in vitro and the changes of ultrastrustures of KCs were observed by transmission electron microscope. The levels of interleukin 6(IL-6), tumor necrosis factor α(TNF-α)and γ-interferon(INF-γ)in the KCs were detected by ELISA. The expression levels of intercellular adhesion molecule 1(ICAM-1) protein in the KCs was detected by Western blotting method. Transwell chamber assay was used to detect the neutrophil chemotaxis ability of the KCs, and the phagocytic ability of KCs was observed under inverted microscope.Results: The number of mitochondria and rough endoplasmic reticulum (RER) in the KCs of the diabetic mice was reduced,the RER expanded,the mitochondria swelled, and the lipid droplets were increased. Compared with non-diabetes group, the levels of IL-6, TNF-α, INF-γ and the expression level of ICAM-1 in the KCs of the mice in diabetes group were significantly increased (P<0.05); the neutrophil chemotaxis ability of the KCs was enhanced (P<0.05), and the phagocytic ability was decreased (P<0.05). Compared with diabetes group, the levels of IL-6, TNF-α, INF-γ and the expression level of ICAM-1 in the KCs in diabetes+metformin group were significantly decreased(P<0.05); the neutrophil chemotaxis ability was decreased (P<0.05), and the phagocytic ability was enhanced (P<0.05).Conclusion: Metformin can inhibit the inflammatory response of KCs in the diabetic mice and improve its phagocytic function and protect the KCs.

Key words: metformin, diabetes mellitus,experiment, Kupffer cells, inflammatory response

CLC Number:

R587.1

WANG Ruibin, ZHAO Yanjie, SONG Qingkun, SHENG Minjia. Inhibitory effect of metformin on inflammatory responseof Kupffer cells of liver in diabetic mice and its improvement on phagocytic function[J].Journal of Jilin University(Medicine Edition), 2019, 45(01): 39-44.

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Inhibitory effect of metformin on inflammatory responseof Kupffer cells of liver in diabetic mice and its improvement on phagocytic function

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