新鲜羊膜对兔宫腔黏连的预防作用及对子宫内膜容受性的改善作用

doi:10.13481/j.1671-587X.20210424

Abstract

Abstract: Objective

To set up the rabbit intrauterine adhesion （IUA） models， and to explore the effect of placing fresh amniotic membrane in the uterine cavity in the prevention of IUA and its improvement effect on the endometrial receptivity.

Methods

Thirty female New Zealand white rabbits were randomly divided into control group， model group and treatment group， with 10 in each group. Using scraping intrauterine mechanical damage to the endometrium and placing cotton lines soaked in lipid polyaccharides （LPS） in the intrauterine cavity， the rabbit IUA models were established in model group and treatment group. For the rabbits in treatment group， fresh amniotic membrane was placed in the uterine cavity at the same time.The control group was a sham operation group with no uterine treatment. The uterine specimens were collected at the 1st and 2nd weeks. The number of glands and the percentages of endometrial fibrosis areas in endometrium tissue of the rats rabbits in various groups were compared by HE and Masson staining， and the expression levels of integrin αVβ3 and leukemia inhibitory （LIF） proteins in endometrium tissue of the rabbits were detected by immunohistochemical staining.

Results

Compared with control group， the number of glands in endometrium tissue of the rabbits in model group and treatment group were decreased significantly at the lst and 2nd weeks （P<0.05），the percentages of endometrial fibrosis areas were increased significantly（P<0.05），and the expression levels of integrin αVβ3 and LIF proteins in endometial tissue were decreased significantly （P<0.05）.Compared with model group， the number of glands in endometrium tissue of the rabbits in treatment group was increased significantly at the 1st and 2nd weeks（P<0.05），the percentages of the endometrial fibrosis areas were decreased significantly （P<0.05）， and the expression levels of integrin αVβ3 and LIF proteins in endometrium tissue were increased significantly （P<0.05）.In control group and treatment group， compared with the 1st week， there were no statistical differences in the number of glands，the percentages of endometrium fibrosis areas， and the expression levels of integrtin αVβ3 and LIF proteins at 2nd week（P>0.05）. But in model group， compared with the 1st week， the percentage of endometrium fibrosis area was decreased significantly at were 2nd week（P<0.01） and the expression levels of integrtin αVβ3 and LIF proteins in endometrium tissue were increased significantly （P<0.05）.

Conclusion

The placement of fresh amniotic membrane in the uterine cavity can reduce the degree of IUA and can improve the endometrial receptivity. The effect is fast and lasting.

Key words: uterine cavity adhesion, fresh amniotic membrane, integrin αVβ3, leukemia inhibitory factor, endometrial receptivity

CLC Number:

R711.74

Yanyan FAN,Na WANG,Wen XU,Yajie GE,Lina WANG. Preventive effect of fresh amniotic membrane on intrauterine adhesion and its improvement effect on endometrial receptivity in rabbits[J].Journal of Jilin University(Medicine Edition), 2021, 47(4): 990-998.

Figures/Tables 8

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References 24

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Group	Number of glands
Group	1st week	2nd week
Control	8.720±0.885	8.750±0.750
Model	3.320±0.668^*	3.750±0.556^*
Teatment	7.200±0.525^*△	7.580±0.577^*△
P	<0.05	<0.05

Group	Percentage of endometrial fibrosis area
Group	1st week	2nd week
Control	16.61±2.75	16.57±2.65
Model	46.68±3.32^*	39.70±2.03^*#
Treatment	20.50±1.92^*△	19.97±3.42^*△
P	<0.01	<0.01

Group	Expression level of integrin αVβ3 protein
Group	1st week	2nd week
Control	5.11±0.18	5.12±0.18
Model	1.89±0.02^*	2.06±0.13^*#
Treatment	4.19±0.14^*△	4.37±0.23^*△
P	<0.01	<0.01

Group	Expression level of LIF protein
Group	1st week	2nd week
Control	5.16±0.24	5.08±0.13
Model	1.97±0.22^*	2.14±0.13^*#
Treatment	4.79±0.15^*△	4.22±0.20^*△
P	<0.05	<0.05

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[4]	JIANG Qiu, AI Yong-hua, NIE Dai-bang, SUN Hong-chen, OUYANG Hong-sheng. Cloning of mouse full-lenth LIF gene and construction of  eukaryotic expression vector pcDNA3.1-LIF [J]. J4, 2008, 34(5): 782-785.
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Preventive effect of fresh amniotic membrane on intrauterine adhesion and its improvement effect on endometrial receptivity in rabbits

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