新型nHA/PLGA纳米复合微球对大鼠颅骨缺损的修复作用

doi:10.13481/j.1671-587X.20220309

Abstract

Abstract: Objective

To investigate the repair effect of new nano-hydroxyapatite （nHA）/polylactide-glycolide （PLGA） nanocomposite microspheres on the skull bone defects of the rats， and to provide the basis for the clinical application of the material.

Methods

Two symmetrical critical bone defect models with a diameter of 5 mm were prepared in the skulls of 9 SD rats with a dental low-speed handpiece， and they were randomly divided into experimental group （implanted with nHA/PLGA nanocomposite microspheres）， control group （implanted with Bio-Oss particles） and blank group （only the defect was prepared without filling material），and there were 6 skull defect models in each group. The CT three dimensional reconstruction of skull was performed on the right postoperative day， 4 weeks after surgery and 8 weeks after surgery to check the repair effect of skull defect.The rats were sacrificed at the 8th week，and micro-computed tomography （micro-CT） scanning was used to scan the three-dimensional reconstruction， and the number of trabecular bone（Tb.N ）， trabecular bone spacing （Tb.Sp）， trabecular bone thickness （Tb.Th）， and the ratios of new bone volume/total bone volume （BV/TV）in skull defect area of the rats in various groups were detected.

Results

The naked eye observation results showed that the boundary of the skull defect area of the rats in experimental group was clear， and new bone could be seen in the intergranular space； in control group， there was granular bone powder accumulation in the skull defect area without obvious absorption； in blank group， there was obvious depression in the central area of skull defect without obvious bone deposition.The CT three-dimensional reconstruction results showed that 8 weeks after sugery，compared with blank group， the rats in experimental group had obvious new bone formation； compared with control group， the rats in experimental group had obvious new bone deposition.The micro-CT detection results showed that 8 weeks after sugery， compared with control group， there were no significant differences in the Tb.N， Tb.Sp and Tb.Th in skull defect area of the rats in experimental group （P>0.05）， and the BV/TV was decreased （P<0.05）； compared with blank group， there were significant differences in the Tb.Sp， Tb.Th and BV/TV in skull defect area of the rats in experimental group and control group （P<0.05）.

Conclusion

nHA/PLGA nanocomposite microspheres have a good repair effect on the bone defects.

Key words: Skull defect, Bone repair, Bio-Oss particles, Bone tissue engineering, Nano-hydroxyapatite/polylactide-glycolide nanocomposite microspheres

CLC Number:

R782

Jie PAN, Dezhou WANG, Wenzhi SONG. Repair effect of new nHA/PLGA nanocomposite microspheres on skull defects of rats[J].Journal of Jilin University(Medicine Edition), 2022, 48(3): 615-621.

Figures/Tables 3

References 30

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Group	Tb.N（mm^－1）	Tb.Sp（l/mm）	Tb.Th（l/mm）	BV/TV（η/%）
Blank	3.613±0.300	0.187±0.020	0.078±0.010	28.007±4.820
Control	3.890±0.200	0.058±0.020^△	0.210±0.010^△	81.286±4.330^△
Experimental	3.768±0.200	0.068±0.020^△	0.191±0.010^△	72.034±5.200^*△

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Repair effect of new nHA/PLGA nanocomposite microspheres on skull defects of rats

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