Abstract: Objective To clone and express the genes of hβ₂GPⅠ-DI and hβ₂GPⅠ-DI₂ in E.coli M15. Methods Target gene β₂GPⅠ-DI was cloned by PCR,the PCR products were connected into dimmer,the monomer and dimmer were inserted into PGEM-T easy vector and sequenced. Then they were inserted into an expression vector PQE30 and expressed in E.coli M15. The recombinant proteins were identified by Western blotting. Results The expected protein of hβ₂GPⅠ-DI and hβ₂GPⅠ-DI₂ recombinant protein were synthesized, their relative molecular mass were 9 000 and 17 000. The two proteins could be recognized by hβ₂GPⅠ mAb with Western blotting. Conclusion The monomer and dimmer of hβ₂GPⅠ first domain gene are constructed successfully.

Key words: sub>GPⅠ, genetic vectors, gene expression