Abstract:

Abstract:Objective
To investigate the effects of ionizing radiation on the expression of γ-H2AX in Jurkat cell lines. Methods The changes of the expressions of γ-H2AX in Jurkat cell lines after X-irradiation with different doses(0.5,1.0,2.0,4.0,and 6.0 Gy) and after irradiation with 2.0 Gy X-rays for different time(0,0.5,1.0,4.0,8.0,16.0,and 24 h) were analyzed by flow cytometry (FCM),and the apoptotic rate of Jurkat cell lines was detected during dose-effect experiment. Results In time-effect experiment,the results showed that the expression of γ-H2AX reached to the peak within 1 h,followed by a time-dependent decrease,kept expressing at 16.0 h,and had no significant difference with control at 24.0 h. In dose-effect experiment,it was demonstrated that the expressions of γ-H2AX showed a dose-dependent increase in the range of 0.5-4.0 Gy at 1.0 h after exposed to X-rays,and dropped at the does of 6.0 Gy;at 8.0 h after exposure to those doses above,the express levels of γ-H2AX enhanced linearly with the dose increased in the range of 0-4.0 Gy,showing a clear dose-dependent manner(P<0.01). Conclusion The γ-H2AX expression could be increased by X-irradiation in time-dependent and dose-dependent manners.

Key words: irradiation;radiation;protein expression;γ-H2AX;Jurkat cells