J4 ›› 2009, Vol. 35 ›› Issue (3): 470-473.
Previous Articles Next Articles
CUI Meng-Ji1, WANG Fang2, GU Chun-Mei1, HUANG Jin-Jie1, NA Guang-Xian3
Received:
Online:
Published:
Abstract:
Abstract:Objective To study the effects of lactate peritoneal dialysis solution(L-PDS) with different concentrations on apoptosis of human peritoneal mesothelial cells(HPMC),the expressions of bcl-2,bax and activity of caspase-3.Methods HPMC were separated using enzyme digestion and cultivated stably in vitro.After HPMC were co-cultivated with different concentrations(1.50%,2.50%,4.25%) L-PDS,flow cytometry was used to test the apoptosis of HPMC,RT-PCR was used to observe the expressions of bcl-2 and bax,fluorometric method was used to detect the activity of caspase-3. Results Compared with control group, L-PDS could induce the apoptosis of HPMC,especially in high concentration.With the increasing of L-PDS concentration,the expression of bcl-2 mRNA decreased,the expression of bax mRNA increased,the activity of caspase-3 raised.There were significant differences of the indexes mentioned above between 4.25%, 2.50% L-PDS groups and control group(P<0.05),there also was significant difference between 4.25% L-PDS group and 2.50% L-PDS group(P<0.05),but there was no significant difference between 1.5% L-PDS group and control group(P>0.05). Conclusion L-PDS could induce HPMC apopto9sis,which may be executed by alternating of the expressions of bcl-2,bax and activating of caspase-3.
Key words: lactate peritoneal dialysis solution;human peritoneal mesothelial cells;apoptosis;genes,bcl-2;bax;caspase-3
CLC Number:
CUI Meng-Ji, WANG Fang, GU Chun-Mei, HUANG Jin-Jie, NA Guang-Xian. Effects of lactate peritoneal dialysis solution on apoptosis of human peritoneal mesothelial cells,expressions of bcl-2,bax and activity of caspase-3[J].J4, 2009, 35(3): 470-473.
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://xuebao.jlu.edu.cn/yxb/EN/
http://xuebao.jlu.edu.cn/yxb/EN/Y2009/V35/I3/470
Cited