Abstract:

Objective: To observe the protective effect of deproteinized extract of calf blood (DECB) on CCl₄-induced liver injury of the mice,and to preliminarily discuss its mechanism.Methods: Fourty healthy ICR mice were randomly divided into control group,model group,positive drug group, and DECB group(n=10).The mice in control group were given saline solution 20 mL·kg^-1 by intragastric administration;the mice in DECB group were given DECB 1 g·kg^-1;the mice in positive drug group were administrated with Hugan Tablets0.63 g·kg^-1;once a day for 30d. 2h after the last administration,the mice in control group were intraperitoneally injected with mixed solution,and the mice in other groups were intraperitoneally injected with 10% CCl₄ (10 mL·kg^-1) mixed solution,then fasted to estabish CCl₄-induced acute injury models. The activities of aspartate aminotransferase (ALT) and alanine transaminase (AST) in serum of the mice in various groups were detected;the levels of total superoxide dismutase (T-SOD),glutathione (GSH) and malonic dialdehyde (MDA) in liver tissue of the mice in various groups were determined. The pathological changes of liver tissue were detected by HE staining and the apoptosis of liver tissue was detected by TUNEL method.Results: The ALT and AST activities in serum of the mice in DECB group were significantly lower than those in model group (P<0.05);compared with model group the MDA and GSH levels in liver tissue of the mice in DECB group were reduced(P<0.05),the T-SOD level was obviously increased(P<0.05);the pathological damage of liver tissue caused by CCl₄ was reduced and the apoptosis index was significantly decreased.Conclusion: DECB plays a protective role in CCl₄ -induced liver injury.It can enhance the antioxidant capacity of liver and inhibit the apoptosis of liver cells.The mechanism may be related to reducing the oxygen free radicals and inhibiting the lipid peroxidation.

Key words: deproteinized extract of calf blood, disease model,anmial, CCl₄, acute liver injury