Abstract:

Abstract:Objective To investigate the differences of metabolites of aconitine in rabbit urine under different routes of administration in order to demonstrate the  effects of different metabolites. Methods With the method of LC/ESI-MSn,15 rabbits were divided into 3 group:1.0 mg•kg^-1 AC lavage administration group,0.02 mg•kg^-1intravenous injection group and physiological saline lavage group (control group),the quasi-molecule particles and each stage fragment ions were detected to identify the metabolites administration  in the urine.Results Compared with control group,one major metabolite (M1) except aconitine (AC) was found in intravenous injection group,and two major metabolites (M1,M2)in lavage administration group.M1 was deduced as 16-O-demethylaconine (M1),M2 exhibited the deoxidation and nor-methyl production of aconitine,and M2 may  be  the metabolic products of AC which lost methylene and hydroxyl.Conclusion Two kinds of metabolites of aconitine are obtained under different routes of administration.Compared with AC,they have correlation with pharmacological and toxicological effects of AC.

Key words: aconitine；metabolites；LC/ESI-MSn