Abstract:Objective To construct TAZ expressing lentivirus vector pLenti6/V5-DEST-TAZ and study the regulatory effect of TAZ on the differentiation of MC3T3-E1 preosteoblasts. Methods LR reaction was performed to clone the pENTR(tm)221-TAZ plasmid containing TAZ cDNA into pLenti6/V5-DEST plasmid.The new recombinant plasmid was identified by restriction enzyme.The overexpression of TAZ in cells was validated by Western blotting.The pseudoviral particles containing the expressed construct were generated by lentiviral packaging system in 293FT cells,which were used to infect MC3T3-E1 to select monoclonal cells by using Blastcidin added.MC3T3-E1 wild type cell line and TAZ overexpressing cell lines were induced towards osteoblasts with condition medium.Differentiation and mineralization of two cell lines were verified by assay of Von Kossa staining and Alizarin red staining,respectively.Results Agarose electrophoresis and sequencing examination showed that TAZ cDNA was cloned into lentiviral vector pLenti6/V5-DEST.The recombinant plasmid could be expressed correctly.The pseudoviral particles with TAZ were obtained and  the MC3T3-E1 cell lines were infected successfully.The Von Kossa staining and Alizarin red staining results showed that the mineralization of TAZ overexpressing cell lines was higher than that of MC3T3-E1 wild type cell line. Conclusion The pLenti6/V5-DEST-TAZ is constructed successfully,and it can express in the cells correctly.The  human TAZ cDNA is cloned into the lentiviral vector pLenti6/V5-DEST. The results indicate that the overexpression of TAZ can promote the differentiation of MC3T3-E1 cell line into osteoblasts.

Abstract:Objective To study the effect of low dose radiation(LDR) on the kidney function and morphology  in C57BL/6J mice with  diabetic nephropathy(DN)induced by streptozotocin(STZ) and illuminate the protective function of LDR on kidney damage caused by diabetes mellitus(DM).Methods The healthy and right age C57BL/6J mice were divided into 4 groups including control,DM,LDR and DM/LDR.The mice in DM and DM/LDR groups were injected intraperitoneally with STZ to set up DM models.The mice in DM/LDR and LDR groups were irradiated with 25 mGy X-rays every other day for 4 weeks.The changes of blood glucose level,urine index level and the morphology of glomerular were detected at 2,4,8,12,16 weeks after radiation.Results The blood glucose levels of mice in DM and DM/LDR groups after STZ-induced DM model preparation were higher than those in LDR and control gourps(P<0.05).After treated with LDR for 2 weeks,the blood glucose level in DM/LDR group was supressed and significantly lower than that in DM group (P<0.05).Moreover the the change  had been kept to 16 weeks.  In addition,compared with DM group,the level of urine micro albumin(MALB) in DM/LDR group was decreased and the urine creatinine(Cre) level was increased.Compared with DM group,the morphological results showed that the glomerular mesangial expansion and mesangial cell proliferation were significantly supressed in DM/LDR group(P<0.05).Conclusion LDR can promote the decease of blood glucose level efficiently,relief the change of kidney fuction,supress and delay the pathological changes of DN.

Abstract:Objective To investigate the relationship between poly(ADP-ribose)glycohydrolase（PARG） and   vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF)  in colorectal carcinoma(CRC) tissues.Methods Immunohistochemical analysis was used to detect poly(ADP-ribose) polymerase(PARP),VEGF and bFGF in 43 cases of human colorectal carcinoma and 10 cases of intestinal mucosa of incisal margin(control group).Western blotting was used to detect the expressions of PARG,PARP,NF-κB,VEGF and bFGF in human Lovo cell line before and after the treatment of PARG inhibitor gallotannin (GLTN).Results The positive rates of PARP,VEGF and bFGF in colorectal carcinoma tissues were 97.67%(42/43),79.07%(34/43),and 81.40%(35/43),respectively;they were significantly higher than those in control group(P<0.05).The expression of PARP had positive correlation with VEGF (r=0.3968,P<0.05) and bFGF (r=0.5610,P<0.05).In GLTN-treated Lovo cell line,the expressions of PARG,PARP,NF-κB,VEGF and bFGF were markedly reduced compared with untreated cells (P<0.01).Conclusion PARG may affect the expression of the factors related to neovascularization in colorectal carcinoma.Its mechanism may be related to the regulation of PARP and affecting the NF-κB activity.

Abstract:Objective To investigate the differences of metabolites of aconitine in rabbit urine under different routes of administration in order to demonstrate the  effects of different metabolites. Methods With the method of LC/ESI-MSn,15 rabbits were divided into 3 group:1.0 mg•kg^-1 AC lavage administration group,0.02 mg•kg^-1intravenous injection group and physiological saline lavage group (control group),the quasi-molecule particles and each stage fragment ions were detected to identify the metabolites administration  in the urine.Results Compared with control group,one major metabolite (M1) except aconitine (AC) was found in intravenous injection group,and two major metabolites (M1,M2)in lavage administration group.M1 was deduced as 16-O-demethylaconine (M1),M2 exhibited the deoxidation and nor-methyl production of aconitine,and M2 may  be  the metabolic products of AC which lost methylene and hydroxyl.Conclusion Two kinds of metabolites of aconitine are obtained under different routes of administration.Compared with AC,they have correlation with pharmacological and toxicological effects of AC.

Abstract:Objective To observe the effect of pregnanolone(PGN) on  suprachiasmatic nucleus(SCN) neurons of rat brain slices,and analyze the  possible mechanism of central sedation and anesthesia effects of PGN. Methods PGN,GABA,and Bic were perfused into the rat brain slices,the extracellular discharge changes of SCN were recorded after perfusion with extracellular discharge record method.Results  After perfusion of PGN,the activity of  SCN neurons was inhibited,the inhibitory rate was (49.72±16.28)%;Co-application of PGN and GABA could enhance the inhibitory effect on SCN neurons,the inhibitory rate was (71.54±19.62)%，there was significant difference between them（P<0.05 ）;pre-application of GABA blockers Bic could block the effects of PGN,the inhibitory rate was (29.85± 6.20)%(P<0.05）.Conclusion PGN may directly affect the SCN neuron membrane excitability.Its role may be related to GABAA receptor on SCN neuron membrane.PGN can increase the combination of GABA and GABAA receptors to enhance the function of GABA.

Abstract:Objective To investigate the effect of miRNA-21 antisense oligonucleotide（ASOND） on the proliferation and apoptosis of bladder cancer cell line T24,and provide theoretical basis for treatment of bladder cancer. Methods AS-miRNA-21 mediated by liposome was transfected into T24 cells. As-miRNA-21 group,transfection nonsense series group and control group were set up.The protein expression level of miRNA-21 of the transfected cells was detected  by Western blotting,and the proliferation and apoptosis of the transfected cells were measured by MTT and FCM.Results The miRNA-21 expression level of the tumor cells transfected with  AS-miRNA-21 was decreased,the cell proliferation was inhibited.The proliferation                        
rates of T24 cells in AS-miRNA-21 group,transfection nonsense series group and control group were 53.6%,92.5%,and 100%,respectively; the relative apoptotic rates were 13.8%,1.9% and 2.7%.There were significant differences of the proliferation rate and apoptotic rates between the three groups（P<0.01）.Conclusion Antisense miRNA-21 has the action of proliferation inhibition and apoptosis enhancement on human bladder cancer cell T24. Inhibiting the expression of miRNA-21 may become a prospective gene therapy for bladder cancer.

Abstract:Objective To investigate the influence and effect of rAV-Tumstatin on T24 cell lines,and provide experimental basis　for further  study on its   mechanism.Methods rAV-Tumstatin　was constructed and the T24 cell lines were infected.The cells were divided into rAV-Tumstatin group,vacant viral infection group and control group.The proliferation and apoptosis of the infected cells were detected  by  MTT and TUNEL.Results The T24 cells  could  be stably  infected  by  rAV-Tumstatin and   expressed the exogenous gene EGFP. The result of TUNEL showed the apoptotic rates in rAV-Tumstatin  group,vacant viral infection group and control groupwere 32.9%,13.5%,and 6.7%,respectively;there were significant differences between  three groups(P<0.01).The result of MTT which detected the proliferation and growth of T24 cells respectively  showed  as  follows: rAV-Tumstatin group,G0/G1 60.3%,S 17.4%,G2/M 21.5%;vacant viral infection group,G0/G1 45.2%,S 29.5%,G2/M 24.8%; control group,G0/G1 43.7%,S 32.8%,G2/M 23.7%;there 　were also 　significant differences between three groups(P<0.05).Conclusion rAV-Tumstatin can inhibit the proliferation and induce the apoptosis of T24 cells,and it is very valuable for the gene therapy of bladder cancer.

Abstract:Objective  To investigate the effects of 125I seed on the growth and programmed cell death of human glioma cell line SHG-44,and clarify the action mechanism of the related genes in this process. Methods SHG-44 glioma cells were cultivated in vitro, and divided into control group and treatment group.The inhibitory effect of 125I on SHG-44 cell proliferation was determined by MTT method. The  morphological changes of SHG-44 cells were examined by  electron microscopy, TUNEL method and Acridine Orange/Ethidium bromide double fluorescent staining. The gene expresion profile was established by using gene chip technique. Results 125I seed had inhibitory effect on  the proliferation of SHG-44 cells cultivated in vitro in a dose and time-dependent manner.The result of MTT showed that the A value of SHG-44 cells treated with 125I seed was significantly decreased with the increasing of radiation dose and prolongation of time.The inhibitory rate of SHG-44 cells after treated with 125I seed for 3 d was 50%,there was significant difference compared with control group(P<0.05). Autophagy was frequently observed under transmission electron microscope in SHG-44　cells.The number of cells at  S phase  was  reduced while the number of cells at  G1 and G2/M phase was increased.Although the apoptosis in SHG-44 cells was increased,but not more than 2%. There were 56 differential expression genes of SHG-44 cells after exposure to 125I including 36  up-regulation genes  and 20   down-regulation genes. Conclusion 125I seed can inhibit the proliferation of SHG-44 glioma cells in a dose-dependent manner by inducing the programmed cell death. There may be non-apoptotic programmed cell death (autophagy) in SHG-44 glioma cell line after induction by 125I at relatively low concentration. These results suggest that the mechanism of 125I-induced proliferation inhibitory effect and apoptosis in SHG-44 cells may be related to the genes of p53 and ATM pathway，C-myc family ,p16 family ,Bcl-2 family，TNF ligand family and TNF receptor family genes.

Abstract:Objective To study the effect of staphylokinase derivation (SakD) on the function of platelet in hyprlipemia rats and its mechanism. Methods 30 Wistar rats were randomly divided into 3 group: SakD group, hyperlipemia group and  normal diet group (n=10). The rats in SakD group received SakD i.v. for 15 times (0.5 mg?kg-1, once every other day) respectively, and the rats in other  two groups received normal saline in the same way after 8 weeks feeding. The blood samples were collected before administration and 24 h after the last injection. Then the platelet adhesion rate and the adenosine diphosphate(ADP)-induced platelet aggregation rates in various groups were detected, the contents of calmodulin (CaM) and thromboxane(TXA2)in platelet were measured with radioimmunoassay. Results Compared with hyperlipemia group,the adhesion platelet function and ADP-induced platelet aggregation of hyperlipemia  rats in SaKD group was significantly decreased(63.5%±6.71% and 44.7%±8.13%,  respectively, P<0.05;91.56%± 9.01% and  81.22%±3.45%, P<0.05), the releasing of platelet TXA2 and CaM content in platelet were significantly reduced (P<0.05 or P<0.01) Conclusion SakD can suppress  the platelet agglutination and adhesion by intervention of different pathways of platelet activation.

Abstract:Objective To construct the human BRCA1 promotor luciferase report gene vector and detect its activity in cells. Methods The BRCA1 promoter from human normal cervix tissues                 
  was amplified by PCR, and was inserted into the luciferase report gene pGL3-basic vector. The amplified DNA sequence was confirmed by sequencing and then the constructed vector was transfected into  HCT116 cells to detect its activity by Premaga Dual-luciferase report gene detection system. Results The recombinant plasmid was tested by gel electrophoresis and sequencing analysis, it was proved that the plasmid included pGL3-basic DNA sequence and PRL regulating sequence.The sequencing results indicated that the amplified sequence was correct, in p53 minus HCT116 cells the number of BRCA1 promoter was increased (P<0.05), and the luciferase activity detection result demonstrated that the constructed vector had the promotor activity.Conclusion The human BRCA1 promotor luciferase report gene vector has been constructed successfully, and it will become essential material for further study on the function of BRCA1 regulation.

Abstract:Objective To construct an interleukin-2 (IL-2) expression plasmid containing RU486 inducible system and study the regulatory effect of RU486 system on IL-2 gene expression.Methods The plasmid pRS17 containing an RU486 inducible system and plasmid pUC57-IL-2 coding mouse IL-2 gene were digested by restriction enzyme Cla Ⅰ,pRS-IL-2 was constructed by inserting IL-2 gene into pRS17.PCR and restriction enzymes were used to identify the recombinant plasmid.SMMC 7721 cells were treated with RU486 at different concentrations following transfection with recombinant plasmid.The recombinant plasmid was administered into the mice by hydrodynamic injection followed by intraperitoneal injection of RU486.The expressions of IL-2 gene in supernatant and serum were measured by using ELISA.Results By the restriction enzyme digestion and PCR analysis,the recombinant plasmid pRS-IL-2 showed the expected bands.The highest level of IL-2 was detected in the supernatant of cells treated with 10-8 mol?L-1 of RU486,which was 1.95-fold higher than that of the cells without RU486 （P<0.001）.The  serum IL-2 level  in mice after stimulation of RU486 was 320-fold higher  than before RU486 stimulation.IL-2 was undetectable in mice without RU486 injection.Conclusion The IL-2 gene expression plasmid containing RU486 inducible system is successfully constructed.The expression of IL-2 gene is dependent on RU486 presence.

Abstract:Objective To evaluate the protective effect of polysaccharides of lentinus edodes (PLE) on immune and anti-oxidative and hematopoietic function of ionizing irradiated mice,and provide theoretical basis for its chinical application.Methods Fifty ICR mice were randomly divided into normal control group(NC),irradiating control group(IC),low dose of PLE irradiating group(800 mg?kg-1),middle dose of PLE irradiating group(1 600 mg?kg-1),and high dose of PLE irradiating group(2 400 mg?kg-1)(n=10).The mice in PLE groups were gaster-poured by PLE for 7 d,the mice in NC group and IC group were gaster-poured by 0.9% sodium chloride solution. The mice in all groups except NC group were irradiated with 2 Gy X-ray on the eighth day.The spleen  index,thymus index,the activity of SOD and content of MDA,the number of WBC,the micronucleus rate of bone marrow polychromalic erythrocytes (PCE)were detected at the 24th hour after radiation.Results Compared with IC group,the  spleen index,thymus index and activity of SOD in high dose of PLE irradiating group were increased markedly(P<0.05); the contents of MDA in  middle and high dose of PLE irradiating groups were increased significantly(P<0.05).The number of WBC in PLE groups was  increased significantly (P<0.05);the micronucleus rates of bone marrow PCE in middle and high dose of PLE irradiating groups were  decreased significantly (P<0.05).Conclusion PLE has an obvious protective effect on damage in  X-ray irradiated mice.

Abstract:Objective To observe the therapeutic effect of attenuated salmonella carrying siRNA-STAT3 plasmid on transplanted prostatic cancer in mice,and clarify the mechanism of siRNA-STAT3 of inducing apoptosis of prostatic cancer cells in mice. Methods The transplanted prostatic cancer  models of mice were built,then  the mice were randomly divided into Mock group, pGC-Si-Scramble group and pGC-Si-STAT3 group. The general status of the mice was observed and the changes of tumor volume were recorded.The expression levels of mRNA and protein of STAT3 and its downstream genes such as Bcl-2,c-Myc, HIF-1,cyclinD1 were analyzed with RT-PCR and THE Western blotting, and the apoptosis of tumor cells was detected with flow cytometry. Results The transplanted prostatic cancer models of the mice were built successfully.Compared with Mock group, the growth of tumor was suppressed obviously and the weights of tumors were lessened in pGC-Si-STAT3 group（P<0.05）.The result of flow cytometry revealed that the  early apoptotic rates in pGC-Si-STAT3 group, pGC-Si-Scramble group and Mock group were （29.1±1.6）%,（14.7±1.4）%,and（8.9±1.8）%, respectively. Compared with Mock group, the mRNA and protein expression levels of STAT3 in pGC-Si-STAT3 group were singnificantly decreased, the mRNA expressions of the downtream genes, such as BcL-2,c-Myc were decreased, and the protein expression levels of HIF-1, cyclinD1 were also decreaaed（P<0.05）.Conclusion The attenuated salmonella carrying SiRNA-STAT3 can inhibit the growth of transplanted prostatic cancer in mice by regulating the expressions of STAT3 downtream genes and inducing the apoptosis of tumor cells.

Abstract:Objective To investigate the effects of  mangiferin hydrasis granule（MHG） on blood glucose,blood fat and insulin in Goto-Kakizaki （GK）model rats and their mechanisms.Methods Thirty-six male GK rats were randomly divided into control group, rosiglitazone group and MHG （20.0，40.0 mg/kg）groups （n=9）.The rats were administered by intragastric administration for 4 weeks .The change of  body weight was observed and the blood glucose，blood fat ，insulin in serum were detected.Results The body weight rats in control group was significantly decreased，and continuely droped during aderministration;compared with control group，the body weights of rats in rosiglitazone group and MHG 20.0,40.0 mg?kg-1 groups were increased(P<0.05).Compared with control group , the contents of blood glucose in rosiglitazone group and MHG（40.0 mg?kg-1）group were decreased（P<0.05 ）.There was no significant difference of the  insulin content between various groups（P＞0.05）.Compared with control group,the contents of triglyceride（TG）and total cholesterol（TC）in rosiglitazone group were decreased（P<0.05）;the content of total cholesterol（TC）in MHG（40.0 mg/kg-1 ）group was decreased（P<0.05）.Conclusion MHG can reduce the risk of diabetes mellitus by down-regulating the contents of blood glucose and blood fat in GK rats．

Abstract:Objective To explore the effect of captopril  orally  administered for long term on learning and memory function of normal mice in order to provide  experiment base for clinical application of captopril.Methods Normal adult mice were divided control group and captopril group.The mice were administered with captopril （11.08—14.92　mg?kg-1?d-1）　for 12 weeks. The effect of captopri on learning and memory was detected with Morris water maze,Avoiding dark,Step-down and  cholinergic system (Ach content,AchE activity).Results Compared with control group.the latency,distance,average speed and starting angle of mice treated with captopril did not change significantly  from the first day to the fourth day;the time in platform，time in platform quadrant，times passing platform ，distance in platform/total distance×100%,starting angle,and average speed also did not change significantly in the fifth day in  Morris water maze （P>0.05）;the error times and the latency of mice treated with  captopril did not decrease  in the second day in Avoiding dark（P>0.05）,the error times of mice treated  with captopril  did not decrease in the first day and in the second day,the latency did not prolong in the second day in Step-down（P>0.05）.The biochemical results showed that the content of  Ach in the brain was significantly increased (P<0.01),the AchE content was also increased(P<0.05) in mice treated with  captopril compared with control group.Conclusion Taking captopril for a long time may promote the function of  learning and memory in normal adult mice.

Abstract:Objective To clone fibroblast growth factor-21(FGF21)［Arg59］ mutant gene, express it in E.coli and purify the expression products, and provide basis   for Pull-down test and further study on human hepatoma in mouse models.  Methods The codon of the 59th Lysine of FGF21 cDNA was replaced by Arginine codon by PCR site-directed mutation. Then the mutant fragment and SUMO fragment were fused by PCR and subcloned into pET20b expression vector to obtain recombinant plasmid pET20b-SUMO-FGF21［Arg59］ . The recombinant plasmid was transformed into E.coil BL21 (DE3) and the  expression was induced by IPTG. The expressed protein was purified by Ni- NTA Agarose, SUMO protease cutting, molecular sieve chromatography and so on.  Results The 546 bp gene fragment was amplified by PCR，and the sequencing result showed there was an aim mutation. The positive clones of pET20b-SUMO-FGF21［Arg59］ was identified by PCR and digestion identification. A  fusion protein whose relative molecular mass was 31 500 was soluably expressed after induction.The  Western blotting result indicated that the purified product was mature FGF21［Arg59］ mutant protein.  Conclusion FGF21［Arg59］ mutant gene is cloned and the fusion gene is expressed in E.coil and purified successfully.

Abstract:Objective To observe the expression and location of  the plasmid  pcDNA3.1/ KDRn3 after transfected into the retina of C57BL/6J mice by using liposome. Methods The KDRn3 gene was amplified by PCR and then  identified by Enzymatic digestion.20 C57BL/6J mice were randomly divided into 5 groups(n＝4),four groups were intravitreally injected with the optimal liposome plasmid mixture,the other was control group. 2,5,7,and 14 d after injection,the localization of pcDNA3.1/ KDRn3 was observed by using immunfluorescence staining method. Results A 900 bp gene part was digested with Xho Ⅰ and BamHⅠwhich agreed with the length of KDRn3.The red fluorescence of KDRn3 protein expressed in the retinal ganglion layer 2 d after injection;after 5 d or 7 d,it expressed both  in the ganglion layer and in the inner layer.After 14 d,it showed the weakened expression.Conclusion Cationic liposome can mediate pcDNA3.1/KDRn3 gene into the retina of the C57BL/6J mice effectively and it can express for a long time.

Abstract:Objective To approach the potential  of differentiation of human placenat derived mesenchymal-like stem cells(HPMSCS )into endothelial cells in vitro.Methods With the agreement of the patients,the HPMSCs were isolated and cultivated from the placenta of the term delivery parturients.The biomorphological changes were observed under inverted microscope.The surface marker was detected by flow cytometry,and through osteoblastic inducing identification and  lypoblastic inducing identification, the multi-directional differentiation potential was defined.The HPMSCs  were treated with  endothelial cell inducing fluid in vitro and induced to differentiate into endothelial cells, and then fluorescent staining was used to dectect the expression of endothelial cell specific  factor Ⅷ (vWF factor).The cell surface marker was detected by flow cytometry after induction.The endothelial cell phenotypes were observed.Results The shape of the cultured HPMSCs changed  from fibroblast-like cells to endothelial-like cells.The expression of the surface marker-vWF was positive  in induced HPMSCs,the expressions of CD31 and CD34  were also positive.Conclusion HPMSCS can be induced to appear the phenotypes of endothelial cells  by this method,which indicates that HPMSCS have the ability to differentiate into endothelial cells in definite conditions in vitro.

Abstract:Objective To explore the biological effects of human hepatocyte growth factor (hHGF) gene expression on CCl4-injured human hepatocytes and rat hepatic stellate cell line and the protective effect of hHGF gene expression on CCl4-injured human normal liver cell line (HL-7702),and   explore the mechanism of apoptosis.Methods The  stably transfected HL-7702 cell clones were divided into four groups:PCI-neo-hHGF  group,   PCI-neo empty vector group,liposome control group,and blank control group.The proliferative activity of cells was determined by MTT method.The protein expressions of hHGF,caspase-3,caspase-8, and caspase-9 were measured using Western blotting in PCI-neo-hHGF transfected HL-7702 cells and CFSC-2G cells.Results After the HL-7702 cells were transfected with PCI-neo-HGF and cultivated for 48 h,the cell proliferation activity was significantly higher than those in  PCI-neo,liposome and control groups (P<0.01);and with the increasing of transfected dose of PCI-neo-HGF,the proliferation activity of the cells had a trend of increasing,but there was no significant difference between various groups(P>0.05).The protein expression of caspase-3 in PCI-neo-HGF transfected HL-7702 cells was decreased significantly compared with those in CCl4-injured HL-7702 cells and PCI-neo transfected HL-7702 cells;the protein expressions of caspase-8 and caspase-9 were not detected;the protein expression of caspase-3 in PCI-neo-HGF transfected CFSC-2G cells was increased compared with those in CCl4-induced CFSC-2G cells,the protein expression of caspase-9 was also positive.Conclusion PCI-neo-HGF can promote the growth and proliferation of HL-7702 cells,and inhibit  the  apoptosis of CCl4-injured HL-7702 cells,and promote the apoptosis of  rat CFSC-2G cells 　in vitro.Its mechanism may be related to the up-regulation of the protein expressions of caspase-3 and caspase -9.

Abstract:Objective  To construct the acute myocardial infarction models in rats with blood stasis and study the difference on blood biochemics between the acute myocardial infarction models  with blood stasis and the simple acute myocardial infarction models.Methods Wistar rats were randomly divded into   control group, acute blood stasis model group,acute myocardial infarction sham operation group, acute myocardial infarction model group and of acute myocardial infarction model with blood stasis group.The acute myocardial infarction models under the status of the acute blood stasis in rats were set up.The serum malondialdehyde (MDA),nitric oxide(NO),free fatty acid (FFA),tumor necrosis factor-α (TNF-α) levels were detected,the activities of serum superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and the levels of prostacycline(PGI2),thromboxane A2 (TXA2) and endothelin (ET) in plasma were determined.Results There were not obvious differences in MDA,SOD,GSH-Px and FFA between the acute myocardial infarction models with blood stasis in rats and the simple acute myocardial infarction models(P<0.05).The decrease levels of PGI2 and NO,and the increase extents of TXA2,ET and TNF-α in the acute myocardial infarction models in rats with blood stasis were  higher than those in the simple acute myocardial infarction models(P<0.05).Conclusion The changes of the myocardial damage induced by oxygen free radical and the metabolic disorder of FFA resulted from the myocardial ischemia are not significant,while the releasing contents of interior active mature,such as ET,FFA,TXA2 and NO,are significant when the acute myocardial infarction models in rats with blood stasis and the simple acute myocardial infarction models are compared.The results show that it is  defective to evaluate pharmacodynamics of traditional Chinese drug with only simple acute myocardial infarction models.

Abstract:Objective To determine the changes of pennation angle that occurred after rotator cuff tears,and find evidence for the functional deterioration of the involved rotator cuff muscles.Methods Twenty embalmed cadaveric shoulders were used for the current study.Ten shoulders with various types of rotator cuff tears (tear group) were compared with other 10 shoulders having intact rotator cuff tendons (control group).The length and width of the tear were measured and the tear area was calculated by multiplying them.After removing the muscles from the scapula,the muscle fibers were removed slice by slice in the plane parallel to the surface of the muscle till the slice which contained the intramuscular tendon was reached.The photographs of this slice were taken and the digital images were analyzed using a computer software to get the pennation angles for each muscle.Then the correlation between the size of the tear and the pennation angles of the supraspinatus muscle and the infraspinatus muscle were analyzed statistically.Results The pennation angles of the supraspinatus and infraspinatus muscles in tear group were significantly greater than those in control group(P<0.05).In tear group,a positive correlation was observed between the pennation angle of the supraspinatus muscle and the tear length (r=0.854,P<0.05).A positive correlation was also observed in tear group between the pennation angle of the infraspinatus muscle and the tear area (r=0.759,P<0.05).Conclusion The pennation angles of the rotator cuff muscles increase with the increasing size of rotator cuff tears.It means that the larger the tear size, the more the function loss.

To study the stabilities of recombinant human keratinocytegrowth factor-2(rhKGF-2) deposited at different conditions,and elucidate the influences of temperature,pH,buffer solutions and repeated freeze thawing on rhKGF-2 stability.Methods rhKGF-2 was deposited at different conditions such as temperatures (－20℃,4℃,25℃,40℃),pH(pH 4－9),buffer solutions(Citrate buffer solution,Disodium hydrogen phosphate-Citrate buffer,Phosphate buffer,Jifeishi buffer) and repeated freeze thawing, respectively.The purity of rhKGF-2 was determined by RP-HPLC,and the appearance was also observed.Results All properties of rhKGF-2 were not altered during the observation period at －20℃ and 4℃;the purity of rhKGF-2  deposied for 28 d was reduced to (68.20±0.14)% compared with 0 h(100.00%)  at 25℃;the purity of rhKGF-2  deposited for 9 h was reduced to (4.80±0.39)% compared with 0 h(100.00%)  at 40℃. The purity of rhKGF-2(pH 6.0) deposited for 90 d was  reduced to(79.20±0.12)% compared with 0 h(100.00%) at 25℃(P<0.05);the purities of rhKGF-2(pH 4.0,9.0) deposited for 120 h were respectively reduced to (15.60±0.3)% and (67.30±0.1)% compared with 0 h(100.00%)at 25℃(P<0.01).The purity of rhKGF-2(pH6.0) deposited in Citrate  buffer solution deposited for 60 d  was  reduced to (79.20±0.15)% compared with 0 h(100.00%)  at 25℃(P<0.01).The purity of rhKGF-2 had no significant change  after repeated freeze thawing(P>0.05).Conclusion rhKGF-2 is stable when deposited at low temperature and pH6.0－7.0 neutral conditions.High temperature,acidic and basic conditions are harmful for rhKGF-2 stability.It is profit for rhKGF-2 stability in Citrate buffer solution.There is no impact on rhKGF-2 stability after repeated freeze thawing.

Abstract:Objective
To investigate the inhibitory effect of siRNA interfering E2F3 gene  on prolifera
tion of bladder cancer 5637 cells,and clarify  the biomechanism of inhibitory effect of   E2F3 gene  silencing  on the proliferation of  5637 cells.Methods There pairs of  nucleotide chains coding short RNA sequence and  targeting E2F3 gene were chemically synthesized and cloned into pRNAT-U6.1/Neo digested  with BamHⅠand Hind Ⅲ． RNAi plasmid(pRNAT-U6.1-E2F3/Neo) was constructed．The reconstructed RNAi plasmids were identified by electrophoresis  and  confirmed by sequencing analysis. The recombinant plasmids were transformed into strain DH5α, and the recombinant pRNAT-U6.1/Neo was transfected into 5637 cells. Before and after transfection, the levels of E2F3 mRNA and E2F3 protein expression were detected by RT-PCR and Western blotting,the  cell cycle was assessed by flow cytometry, the  apoptotic rate was detected by Annexin-V/PS kit. Results Compared with control group,the  E2F3 gene expression was markedly down-regulated in 5637 cells following RNA interference treatment( P<0.001), the percentage of cells at G1 phase and the apoptotic rate were up-regulated in transfection cells group,there were significant difference between RNAi cells groups and control group（P<0.001）.  Conclusion siRNA can significantly  inhibit the E2F3 gene expression in bladder cancer 5637 cells, decrease cell proliferation , arrest cell cycle and increase apoptotic rate.

Abstract:Objective
 To approach the spectrum of pathogen of candidal vaginitis and analyze the randomly amplified polymorphism DNA (RAPD) of isolated pathogen,and provide basis for  clinical diagnosis and epidemiological surveillance of candidal vaginitis.Methods The specimens of vaginal discharge of 106 patients suspected with candidal vaginitis were collected.Pathogenic fungal were isolated and identified.10 random primers (RAPD 1-10) were used to make RAPD analysis on the isolates.Results ①72 strains of Candida were isolated,among which C.albicans was 56 strains (77.78%),non-Candida albicans Candida (NCAC) species was 16 strains (22.22%) including 6 strains of C. glabrata (8.33%),4 strains of C. tropicalis (5.56%),1 strains of C. krusei (1.39%) and 5 strains of other Candida sp. (6.94%).②RAPD analysis result showed that 2 primers (RAPD2 and RAPD5) could have better clear and stabile specific pattern bandings with evident interspecies genetic variability and intraspecies genetic resemblance.Conclusion  C.albicans is the main pathogen of candidal vaginitis.RAPD2 and RAPD5 are suitable for identification and typing of C.albicans and C.tropicalis.

To explore the role and possible mechanism of abnormal activation of STAT3 gene during the occurrence and development of human hepatocellular carcinomar（HCC）.Methods Immunohistochemistry dyeing，RT-PCR and Western blotting were used to detect the mRNA and protein expressions of STAT3
 

gene in HCC cell lines SMMC7721,Bel7402 and HepG2 and human HCC tissues.Results There were high expressions of STAT3 gene in SMMC7721，Bel7402 and HepG2 cells,and there was no significant difference among them（P>0.05）.There were high expressions of STAT3 gene at the mRNA and protein levels in HCC tissues and tissues surrounding carcinoma,and there was significant difference between normal tissues and HCC tissues（P<0.05）,while there was no difference between HCC tissues and tissues surrounding carcinoma（P>0.05）.The mRNA and protein expressions of VEGF,survivin and c-myc genes were increased in HCC tissues,while the mRNA and protein expressions of p53 were decreased in HCC tissues.Conclusion The persistent activation of STAT3 gene may occur at early stage of HCC pathogenesis and plays an important promoting role in the carcinogenesis of HCC.

To study the expression of β-catenin in ovarian serous cystadenocarcinoma tissues and disscuss the clinical significances of β-catenin in the diagnosis of ovarian serous cystadenocarcinoma.Methods RT-PCR method was used to detect the expressions of β-catenin mRNA in tissue samples of 36 ovarian serous cystadenocarcinoma,10 ovarian serous cystadenoma and 10 normal ovary tissues.The expressions of β-catenin in 60 ovarian serous cystadenocarcinoma,24 ovarian serous cystadenoma and 24 normol ovarian tissues were detected by immunohistochemistry.Results The expression level of β-catenin mRNA in ovarian serous cystadenocarcinoma tissues（1.001±0.192）was significantly higher than those in normal ovarian tissues（0.588±0.128）and ovarian serous cystadenoma tissues（0.600±0.262）（P＜0.05）.The expression of β-catenin was mainly located in cytoplasm;the positive rate of β-catenin in ovarian serous cystadenocarcinoma group was 88.33%,which was significantly higher than those in normal ovarian group(20.83%)and ovarian serous cystadenoma group(33.33%) (P<0.05).There was significant difference in the expression level of β-catenin in ovarian cancer between different clinical stages,while the positive rate in stage Ⅰ was 77.77% and in stage Ⅳ was 93.87%(P<0.05);however,there was no significant difference in the expression level of β-catenin in ovarian cancer between different pathlogical grades,while the positive rates in ovarian cancer for G1,G2 and G3 were 85.00%,89.28%,and 91.63%， respectively;there was no significant difference between various groups(P>0.05).Conclusion The expression level of β-catenin in ovarian cancer is higher,the expression level of β-catenin is associated with the clinical staging of ovarian cancer but unrelated with pathological grade;β-catenin may play an important role in the detection and diagnosis of   ovarian cancer.

To detect the serum human kallikrein 10 (hk10) expression in patients with ovarian cancer and control groups，and investigate the clinical significance of hk10 in the diagnosis of ovarian cancer. Methods Enzyme-linked immunoassay （ELISA）mothod was used to detect the hk10 levels in serum in ovarian cancer group(n=50),benign ovarian tumor group(n=20),benign gynecological disease (higher CA125 values,n=26),other malignant gynecological cancer group(n=18) and healthy controls(n=36). Results The level of serum hk10 in the ovarian cancer group was(15.60±9.23) μg.L^-1 and the positive rate was 88.89%,in benign ovarian tumor group they were(6.60±2.65) μg.L^-1 and 10.00%;in other malignant gynecological cancer group they were(6.11±2.42) μg?L-1 and 11.11%,in healthy control group they were(6.00±2.26) μg.L^-1 and 11.11%，the level of serum hk10 and the positive rate in ovarian cancer group were higher than those in other groups(P<0.01).The level of serum hk10 and the positive rate in stage Ⅲ/Ⅳ ovarian cancer group were(18.29±9.74) μg?L-1 and 94.44%,they were significantly higher than those in stage Ⅰ / Ⅱ ovarian cancer group(9.87 μg?L-1±4.04 μg.L^-1 and 75.00%)(P<0.01).The level of serum hK10 in patients with ovarian cancer after operation was (13.83±1.56)  μg.L^-1 which was lower than before operation.Conclusion The serum hk10 level is significantly increased in ovarian cancer group,its specificity was higher than CA125. hk10 is expected to become a new marker in ovarian cancer.

Abstract:Objective
To detect the level of serum  human epididymis protein 4(HE4) in the  patients  with ovarian cancer and approach the significance of serum  HE4  in the diagnosis
of  ovarian cancer.Methods ELISA mothod was used to detect the levels of serum  HE4   in  ovarian cancer group,benign ovarian tumor group,other malignant gynecology tumor group and malignant tumor of other organs group.The changes of the serum HE4 level   in ovarian cancer group before and  after operation were detected.The effects of  the serum HE4 level and CA215 level  in  ovarian cancer group    in the diagnois of  ovarian cancer were compared.Results The serum  HE4 levels ovarian cancer group was (242.76±113.40)pmol.L^-1 and the positive rate was 70.59％,they were higher than those in  benign ovarian tumor group（85.57 pmol.L^-1±63.40 pmol.L^-1,7.02％）,other malignant gynecology tumor group （73.16 pmol.L^-1±12.27 pmol.L^-1,0.00％），malignant tumor of other organs group（80.65 pmol?L-1±16.24 pmol.L^-1,0.00％）and control group（43.95　pmol.L^-1±18.16 pmol?L-1,0.00％）（P<0.01）;the serum HE4 level in  ovarian cancer group after operation(134.83 pmol.L^-1±56.20 pmol?L-1) was lower than  before operation（242.76 pmol.L^-1±113.40 pmol.L^-1）（P<0.05）.The positive  rate of serum HE4  in the early  ovarian cancer  was higher than that of seum CA125.The sensitiveity of HE4 combined with CA125 was  higher  than each of them,the specificity of HE4 combined with CA125 was  lower than each of them(P<0.05).Conclusion The HE4 serum has important significance in the diagnosis of  ovarian cancer,especially，its sensitivity and specificity are all better than CA125 in the diagnosis of  early  ovarian cancer,the detection of  HE4 combined with CA125 can elevate the early diagnosis rate of  ovarian cancer.

Abstract:Objective
To study the expression of Brg1 in laryngeal squamous cell carcinoma (LSCC) tissue and explore its relationship with the occurrence and development of laryngeal cancer.  Methods Immunohistochemical method was used to detect the expressions of Brg1 in 30 cases of LSCC  and 10 cases of paracarcinoma normal mucosa. Results  The expression of Brg1 in paracarcinoma normal mucosa  was positive(the grey value was  124.60±4.51),and the expression of Brg1 in LSCC was low(the grey value was 159.20±7.69),the difference between  two groups was significant（P<0.01）. The　grey values expressed by Brg1 in well,moderately and poorly differentiated LSCC were 132.80±1.92,165.20±2.59,and 179.60±1.52,respectively;there were significant differences betwen moderately,poorly differentiated LSCC and  well differentiated LSCC (P<0.01). The grey values expressed by Brg1 in stage Ⅰ-Ⅱ,stage Ⅲ  and stage  Ⅳ  LSCC were 145.30±2.36,165.20±4.31,and 179.4±3.03,respectively;there were significant differences between stage Ⅲ,stage Ⅳ and  stage  Ⅰ-Ⅱ （P＜0.05）.The grey values expressed by Brg1 in vascular invasion group and no vascular invasion group were 176.80±3.48 and 143.50±8.26,the difference between  two groups was significant（P<0.01）. The grey values expressed by Brg1 in lymph node metastasis group and no lymph node metastasis group were 155.60±4.02 and  161.70±3.52,there was no significant difference between  two groups(P>0.05).   Conclusion  The expression of Brg1 in LSCC is low,and it is related to the development of cancer and pathological factors,which may be an important factor in laryngeal cancer.

Abstract:Objective
To examine the expression of insulin-like growth factor receptor 1（IGFR-1） in breast cancer tissue and its relationship with clinical parameters. Methods The expressions of IGFR-1 were detected in paraffin-embedded specimens from 34 patients with breast cancer and 28 controls with surgically proven benign breast disease with immunohistochemical method. Results The expression rate of IGFR-1 in breast cancer tissue was 76.5%,it was significantly higher than that in benign tissue (44.1%)（P<0.05）.In cancer group,there was positive correlation between the expression level of IGFR-1 and estrogen receptor (ER) and progesterone receptor (PR) (r=0.56,r=0.51,P<0.01);there was no significant relationship between IGFR-1 expression and armpit lymphatic node metastasis and clinical stages (r=0.31,r=0.18,both P>0.05).Conclusion IGFR-1 can express in most of the breast cancer tissues which  could be used as a biomarker for diagnosis and prognosis of breast cancer and   a new target for  treatment of breast cancer.

Abstract:Objective
To explore the effect of  aveolar ridge resorption on the stress distribution of abutment and peridontal tissue in attachment denture,and provide evidence for the application of attachment denture in clinic. Methods The stress ditribution of abutment and peridontal tissue of attachment dentures with aveolar ridge resorption  of abutment at different levels  were measured respectively,and  were compared with cantilever fixed bridge by three-dimensional finit element method.Results The stress peach of abutment and peridontal tissue of cantilever fixed bridge was （3.63E+5）Mpa.The stress peach of abutment and peridontal tissue of attachment dentures was (1.27E+5)Mpa with normal aveolar ridge and it was  lower than that of cantilever fixed bridge;the peach of abutment and peridontal tissue was(3.87E+5)Mpa when aveolar ridge was absorbed by 1/3,and it was lihgtly higher  than that of cantilever fixed bridge,;the peach was (1.38E+6)Mpa when aveolar ridge was absorbed by 1/2,and it was significantly higher than that of cantilever fixed bridge.Conclusion The extralcolonal attachment dentures could decrease the stress of abutment and peridontal tissue,the more the aveolar ridge of abutment is absorbed,the greater the stress of abutment is;when the aveolar ridge of abutment was normal,it is suitable to retore mandibular  single distal -extention absence with extralcolonal denture of C4C5 abutment designed singly,when the aveolar ridge of abutment is absorbed by 1/3,the method  should be used carefully,it is necessary to decrease  lateral forces;when the aveolar ridge of abutment is absorbed by 1/2,the method is not suitable and it is necessary to increase the number of abutmenta and use bilateral design.

Abstract:Objective
To observe the therapeutic effect of   arsenic trioxide(ATO) in combination with   all-trans retinoic acid(ATRA) in treatment of newly diagnosed acute promyelocytic leukemia (APL),and analyze the influencing factors of induction treatment.Methods 107 newly diagnosed APL patients treated by both ATO and ATRA  were divided into high risk,medium risk,low risk,coarse granular(M3a),fine granular(M3b),L-type isoform ,S-type isoform typical karytype,additional karyotype groups according to different risk ranks,morphological  subtypes，fusion gene types and different chromosome types.The early mortality,complete remission(CR) rate,and CR  achieving time  of each group were detected,the prognostic factors influencing the therapeutical effect were determined.Results ①10 of all 107 patients died in early stage,the early mortality was 9.35%,the most common adverse reactions were disorder of coagulation and leukocytosis.②High risk group had obvious differences with medium and low risk groups in early mortality,CR rate and CR achieving time(P>0.05),but there was no difference between the latter two groups(P>0.05). M3b group had longer CR achieving time than M3a group,but they had the same CR rate (P>0.05).The CR rate in (L＋) group was higher than that in (S＋) group. Typical karyotype group had the same CR rate (P>0.05) and CR achieving time as the additional karyotype group (P>0.05).
Conclusion Initial WBC count,morphological subtypes,fusion gene types are important influencing factors for CR in patients with newly diagnosed APL treated by    ATRA in combination with  ATO.However,additional karyotype has little impact on short ter
m CR.

Abstract:Objective
To discuss the curative effect of PEG-interferon α-2a combined with ribavirin in treatment of chronic hepatitis C,and clarify the feasibility of combined therapy in treatment of chronic hepatitis C.Methods 86 cases of chronic hepatitis C patients were divided into treatment group (n=38) and  control group(n= 48).The patients in treatment group were treated with polyethylene glycol α-2a interferon 180 μg,subcutaneous injection once a week,combined with ribavirin 1 000 mg,once daily oral administration,a total of 2 weeks of treatment;the patients in control group were given α-1b interferon 500 000 U intramuscularly once a day,combined with ribavirin 1 000 mg,once daily oral administration,for 24 weeks.According to pre-treatment ALT values,all the patients were divided into low ALT group (more than normal but less than or equal to three times the upper limit of normal) and high ALT group (more than 3 times the upper limit of normal but less than or equal to 10 times the upper limit
 of normal),and the virology,biochemistry,genotyping  before treatment,12 and 24 weeks after treatment,24 weeks after withdrawl were detected and compared  between two groups,as well as ALT,HCV RNA quantitative level  and virological response.Results Virological and biochemical complete response results showed   the efficacies in the treatment group were significantly better than those in control group 24 weeks after treatment and 24 weeks after withdrawl(P<0.05);the virological complete response rate in high ALT group was higher than that in low ALT group(P<0.05);the ALT level had no significant impact on the virological complete response in treatment group,the RNA quantitative level of HCV had no significant impact  on the virological complete response in control group and treatment group(P>0.05).The study of the effect of genotype on the virology indicated that there was significant difference between 1a group and 2a group at the end of treatment (P<0.05),but there was no significant difference between the other groups(P>0.05).The adverse reactions found in two groups were similar,and there was no significant difference. Conclusion The long-acting combination of pegylated interferon and ribavirin  in treatment for chronic hepatitis C is a very effective,and it can significantly increase the efficacy than general interferon.

Abstract:Objective
To analysis the clinical distribution and resistance characteristics in isolates of Acinetobacter baumannii(Ab), and provide evidence for clinical prevention and therapy. Methods 1 504 Ab strains were isolated from clinical specimens in Xuanwu Hospital of Beijing Capital Medical Univercity from January, 2006 to October, 2009. The clinical distribution and the resistance results of Ab were analyzed retrospectively. Results The majority of these Ab strains was distributed in ICUs(665 strains, account for 44.2%) and emergency wards(123 strains, account for 8.2%). Other common wards isolated 716 strains(account for 47.6%). The majority of these Ab strains was isolated from sputum(1 304 strains, account for 89.6%). The resistance of Ab showed the rising tendency to all kinds of antibiotics, especially to imipenem. Despite the resistance rate of imipenem acquired 71.8% in 2009, it was the most sensitive drug of treating Ab(the sensitivity was 28%).The resistance rate of cefotaxime(CTX) was 100% in 2006, whose application was decreased in 2007, but decrea
sed to 33.3% in 2008. Multi-drug resistance Ab had been emerged. Conclusion The majority of Ab distributes in ICU.Carbapenems are the most sensitive drugs of treating Ab. If a highly resistant drug is stopped using over a period of time, the sensitivity of  Ab to antibiotic  may be increased.

Abstract:Objective
To compare the efficacy between  two different methods of internal fixation  in the treatment of senile osteoporotic complicated fractures of  proximal humerus.  Methods
125 senile patients with osteoporotic complicated fracture were divided into two groups to receive different internal fixation and surgical treatment （Kirschner needle or traditional internal fixation was Group A, internal fixation of locking compression plate was Group B, and there were two respective subsections). There were 42 patients with Ⅲ-part fracture according to Neer classification receiving Kirschner needle or traditional internal fixation(Group A1), 29 patients with Ⅲ-part fracture receiving internal fixation of locking compression plate(Group B1). There were 19 patients with Ⅳ-part fracture receiving Kirschner needle or traditional internal fixation(Group A2), 35 patients with Ⅳ-part fracture receiving internal fixation of locking compression plate(Group B2).The patients were  followed up for 6 to 24 months (average 12.2 months).Neer score system was used to appraise therapeutic effect, and 80 points was defined as excellent and good. Results There were 29 cases exceeding or equaling to 80 points in Group A1, the excellent and good rate was 69.05%, while the patients in Group B1 were 26 and the excellent and good rate was 89.66% , there was significant difference between two groups (P<0.05). There were 8 patients exceeding or equaling to 80 points in Group A2, the excellent and good rate was 42.11%, while the patients in Group B2 were 25  and the excellent and good rate was 71.43%,there was significant difference between two groups (P<0.05). Conclusion   Compared with traditional fixation, locking compression plate has advantage in treatment of  senile osteoporotic complicated fractures of  proximal humerus

Abstract:Objective
To assess the long-axis regional systolic function of the left ventricle (LV) in patients with type 2 diabetes mellitus ( T2DM) using two-dimensional strain echocardiography（2D-SE）and explore clinical application value of 2D-SE.Methods 30 T2DM patients with normal LV ejection fraction (LVEF≥55%), 30 normal controls were selected.The peak strain of segments in apical long-axis, four-chamber,and two-chamber views were evaluated,and average peak strain in the long axis at basal,middle and apical levels were assessed by automated functional imaging(AFI).Results LVEDV,LVESV,SV and LVEF in T2DM patients and normal  controls were all within normal limits.Furthermore,there was no significant difference between the two groups (P＞0.05).Compared with normal controls, the peak strain at each plane in T2DM patients was significantly reduced (P<0.01).Conclusion The long-axis regional systolic function of  LV in the patients with T2DM is impaired ahead of global systolic function disturbance.     2D-SE may be used to identify early abnormalities of systolic function in T2DM patients with normal LVEF.

Abstract:Objective
To explore the relative factors of low bone mineral density in old women in order to provide a theoretical basis for early prevention and treatment for osteoporosis in old women. Methods Using dual-energy    X-ray   absorptiometry (DEXA),the ulna and radius bone mineral densities of 915 old women who were 60-74 year-old women retired workers of China First Automobile Works were determined. Questionnaire was divided into age,the intakes of rice,coarse food grain,marine products,milk,acidophilous milk,coffee,bean milk,flour,eggs,fruit,whether smoking or not,whether drinking or not and years of menopause,the body height and the body weight were measured at the same time. Results Age,whether take in flour,eggs and fruit frequently,whether smoking or not,postmenopausal years and body mass index(BMI) were correlated with bone mineral density(P<0.05);multiple gradual regressive analysis demonstrated that  the age was negatively correlated with bone mineral density(B=－0.440,P<0.05),the postmenopausal year  was negatively correlated with bone mineral density(B=－0.314,P<0.05),smoking was negatively correlated with bone mineral density(B=－0.650,P<0.05). BMI was positively correlated with bone mineral density(B=0.959,P<0.05).Conclusion Old age,long post-menopausal years,low BMI,and smoking are relative factors of primary osteoporosis in old women aged 60-74 years.

Abstract:Objective
To evaluate the implementation results of World Bank Loaned TB Control Project,and provide theoetical basis for prevention and treatment of TB.Methods The implementation quarterly reports from 1 April,2003 to 3 March,2008  were collected，and the DOTS cover rate,case detection rate of new smear positive cases and the cure rate of smear positive cases were analyzed,the effects of World Bank Loaned TB Control Project in Qiqihar were evaluated.Results The implementation was fully carried out in Qiqihar,and the DOTS cover rate was 100%. 67 452 suspects who with TB symptoms made clinical consultation to TB dispensaries. 14 453 smear positive cases had been detected.The notification rate of new smear positive cases was 35.6/100000.The case detection rate of new smear positive cases was 70.1%.The cure rate of new smear positive cases reached 92.6%,the cure rate of retreatment smear positive cases was 86.4%. Qiqihar implementation had reached WHO 2010 targets in advance.Conclusion The implementation of World Bank Loaned TB Control Project has reached the goals of the TB Control Project.

Abstract:Objective
To analyze the relation ship between the quality of drinking water and the onset of urolithiasis  in Jilin province and discuss its genesis and prevention strategies. Methods According to the distribution of urolithiasis  in different areas of Jilin province,the contents of main trace elements and main anions of the drinking water from different places were detected. The relationship  between the quality of drinking water and urolithiasis  was analyzed and the method to improve the quality of drinking water was suggested. Results There were obvious differences of the pH,Na+,Ca+ and HCO-3 of drinking water between different areas (P<0.05),and the levels of pH and anions in the area with high morbidity of urolithiasis  were higher. The relation coefficient between pH of drinking water and urolithiasis  was 0.781,and the relation coefficient between anions of drinking water and urolithiasis  was 0.707. It indicates  that there were some relations between the quality of drinking water and the onset of urolithiasis in Jilin province. Conclusion If the water has more calcium,sodium and high pH value, it will be easy to form urinary stone. So it must be purified before drinking.