Abstract:

Abstract:Objective To observe the expression and location of  the plasmid  pcDNA3.1/ KDRn3 after transfected into the retina of C57BL/6J mice by using liposome. Methods The KDRn3 gene was amplified by PCR and then  identified by Enzymatic digestion.20 C57BL/6J mice were randomly divided into 5 groups(n＝4),four groups were intravitreally injected with the optimal liposome plasmid mixture,the other was control group. 2,5,7,and 14 d after injection,the localization of pcDNA3.1/ KDRn3 was observed by using immunfluorescence staining method. Results A 900 bp gene part was digested with Xho Ⅰ and BamHⅠwhich agreed with the length of KDRn3.The red fluorescence of KDRn3 protein expressed in the retinal ganglion layer 2 d after injection;after 5 d or 7 d,it expressed both  in the ganglion layer and in the inner layer.After 14 d,it showed the weakened expression.Conclusion Cationic liposome can mediate pcDNA3.1/KDRn3 gene into the retina of the C57BL/6J mice effectively and it can express for a long time.

Key words: VEGF receptor;gene transfection;retina