Abstract:

Abstract:Objective To investigate the protective effect of Gross Saponin Tribulus Terrestris (GSTT) on the rat myocardial ischemia-reperfusion models and its mechanism.Methods Thirty-six male Wistar rats were divided randomly into sham group,ischemia-reperfusion model group,Xue-Sai-Tong(20　mg·kg-1) group and GSTT (30.0，15.0，7.50 mg·kg-1) groups(n=6).The myocardial ischemia-reperfusion model was induced by 30 min left anterior descending coronary occulusion and 120 min reperfusion in openchest anesthetized rats.The cardiocyte morphological changes were  observed and the contents of SOD,MDA,CK,AST,LDH and NO in serum were detected.Results Compared with sham group,the cardiocytes had serious edema and cytoplasm was stained slightly and nucleus were contracted and strained deeply after reperfusion in model group.In GSTT (30 and 15 mg·kg-1) groups and Xue-Sai-Tong group the  cellular edema was reduced,the cadiocytes maintained normal  appearance.Compared with sham group,the contents of AST,LDH,CK and MDA in model group were increased but SOD and NO were decreased（P<0.01）.Compared with model group,in GSTT（30 and 15 mg·kg-1）　groups and Xue-Sai-Tong group  the contents of MDA,AST,LDH and CK were decreased,and  the contents of SOD and NO were increased（P<0.05 or P<0.01）.Conclusion GSTT has the protective effects on myocardial ischemia-reperfusion injury in rats,which may be related to inhibition of lipid oxidation and modulation of endogenous antioxidant enzyme activities.

Key words: Gross Sapoini； Tribulus Trerrestris;myocardial ischemia;reperfusion injury；anti -free radical