Abstract:

Abstract:Objective To study the inhibitory effects of triethyltin chloride (TETC) on proliferation and the morphological changes of rat C6 glioma cells in vivo.Methods C6 glioma cells were autotransplanted into 16 Wistar rats,and the rats were divided into experiment group and control group.TETC was injected into each rat through intraperitoneal route at the dose of 1 mg·kg^-1·d^-1 and the injection lasted 4 d in experiment group,and the physiological saline was injected into each rat in the same way as experiment group in control group.The weights of C6 glioma were measured and the proliferation rate of the tumor was calculated after 14 d.The morphological changes of C6 glioma cells were observed by HE staining and electron microscope.Results The weight of C6 glioma in TETC group was lighter than that in control group(P<0.01),and the proliferation rate was decreased compared with control group (P<0.01).The cell number and cell density in TETC group were lower than those in control group observed by HE staining and the chromatin margination could be observed  in  TETC group.Conclusion TETC can inhibit the proliferation of rat C6 glioma cells in vivo and the mechanism of which may be involved in apoptosis.

Key words: triethyltin chloride;glioma;cell proliferation;apoptosis