Abstract:

Abstract:Objective To improve the administration method of lentiviral vector in rat CA1 region of hippocampus by electrode pipe installation and detect its validity and security.Methods Fourty-five male healthy SD rats were randomly divided into three groups: sham operation group(SH),normal saline group (NS),GFP gene lentiviral vector injection group(eGFP).The CA1 region was located by stereotaxic instruments and the lentiviral vector was administered by electrode pipe installation.At day 7,14 and 30 after the injection,SD rats were frozen sectioned and the expression of the green fluorescent protein was detected by fluorescence microscope.Nissl staining was used to detect the hippocampus injury.Results The stable expression of the green fluorescent protein was detected in rat CA1 region of hippocampus at day 7,14 and 30 using this method and the number of survival neurons had no significant difference between three groups (P>0.05).Conclusion The method of electrode pipe installation to inject lentiviral vector in rat hippocampus is successfully established and the high level expression of fluorescent protein can be detected with no significant injury,which lays the basis for its application in the gene therapy of the neurodamaged diseases.

Key words: lentiviral vector;genes;hippocampus;green flurescent protein