Abstract:

Abstract:Objective To set up H9C2 myocardial cell hyertrophy model with angiotensin Ⅱ(Ang Ⅱ) and  observe the effect of CLC-2 on the hypertrophy process of H9C2 myocardial cells and discuss its mechanism. Methods The H9C2 cells were divided into control group,AngⅡ group,candesartan group,and candesartan plus AngⅡ (candesartan + AngⅡ) group. The changes of H9C2 cell volume in various groups were detected by flow cytometry. The changes of CLC-2 mRNA and protein expressions were detected by RT-PCR and Western blotting methods. Results Compared with control group,the percent of the cells with large volume and the expressions of CLC-2 mRNA and protein in AngⅡ group were increased significantly(P<0.01). Compared with AngⅡ group, the percent of the  cells with large volume and the expression levels  of CLC-2 mRNA and protein in candesartan+AngⅡ group were decreased(P<0.01).
Conclusion AngⅡ can induce hypertrophy of H9C2 myocardial cells,and the mechanism may be related with that CLC-2 can affect the concentration of intracellular chloride.

Key words: voltage-gated chloride channels 2;cardiac hypertrophy;angiotensin Ⅱ