Abstract:Objective To investigate the effect of low dose irradiation on the expression changes  of Cyt c and caspase-3 in mouse testis,and  interpret the effects  on  apoptosis in mouse testis induced by irradiation. Methods The mice were divided into different doses（0,0.050,0.075,0.100and 0.200 Gy）groups and different time course (0,6,12 and 24 h) groups. The expressions of Cyt c and caspase-3 mRNA and proteins in mouse testis tissues irradiated with different doses X-rays were observed by real time PCR and Western blotting.Results The real time PCR results indicated that the expressions of Cyt c and caspase-3mRNA were increased with the dose increasing 12 h after irradiation with different doses of X-rays,and the expression of Cyt c mRNA reached the peak after irradiated with 0.100 Gy,but the expression of caspase-3 mRNA expression reached the peak after irradiated with 0.075 Gy;the expression levels of Cyt c and caspase-3protein were the highest when irradiated with 0.075 Gy X-rays. The expressions of Ct c and caspase-3 mRNA after irradiation with 0.075 Gy X-rays were increased with the time prolongation,and reached the peak after 24 h,but the Cyt c and caspase-3 proteins expreesed  the most 12 h after irradiation with 0.075 GyX-rays. Conclusion The mRNA and protein expressions of Cyt c and caspase-3 in mouse testis spermatogenic cells can be increased by low dose irradiation with dose- and time-effect regularity.

Abstract:Objective To construct the Cre rebombinase expression vector and identify its function of recognizing loxP sites and provide the basis for establishment of the animal models of human diseases. Methods The recombinant vectors Cre-pCEP4 and pStop-eGFP were constructed. The MCF-7 cells and porcine embryonic fibroblasts (PEF) were transfected by Fugene HD. The expression of eGFP was analyzed by fluorescence microscope. Results The recombinant vectors Cre-pCEP4 and pStop-eGFP were constructed successfully and they were co-transfected into PEF.The  MCF-7 cells with stable expression of Cre recombinase were transfected with pStop-eGFP after selection of hygromycin B. There were green fluorescent protein exrpression of eGFP analyzed by fluorescence microscope after transfected by the two vectors. No eGFP positive cell was detected in MCF-7 cells and PEF transfected with pStop-eGFP only. Conclusion The recombinant vector Cre-pCEP4 can express Cre recombinase which can recognize loxP sites and delete the DNA agment between two loxP sites in the same orientation.

Objective To observe the effects of transplantation of amniotic epithelial cells(AECs) on nerve regeneration after spinal cord hemisected injury in rats and provide experimental basis for repair of SCI with AECs.Methods The models of spinal cord hemisection-injure were established with 30 male Wistar rats. The gelatin sponge with or without  AECs were respectively implanted into the hemisectedadult rat spinal cord. The SCI model group and the  sham operation group were established as control groups. Two  or four  weeks after the transplantation,the graft/spinal cord tissue structures were observed by routine histological methods;the  neural filament( NF),serotonin (5-HT),calcitonin gene related peptide (CGRP)-positive nerve fibers and glial fibrillary acidic protein (GFAP)-positive cells in graft / spinal cord were observed by immunofluorescence staining. Fluororuby tracing method was used to observe the regeneration of the nerve fibers in the transplants. The BBB test was used to evaluate the  motorfunctional recoveration of the  hind limbs in rats. Results AECs transplantation promoted the regeneration of nerve fibers in the spinal cord itself,and promoted the regeneration of the NF,5-HT,CGRP positive nerve fibers. The regenerating nerve fibers extended through the GFAP-positive area and went into the grafts with AECs.The number of NF positive never fibers in grafts in AECs transplantion group was more than that in gelatinsponge group(P<0.05). There were significant differences of the BBB scores between AECs transplantation group,gelatin sponge group,SCI group and sham operation group(P<0.05).The BBB scores showed no significant differences in functional recovery between AECs transplantion group and gelation sponge and SCI,groups(P>0.05). Conclusion AECs transplantation can promote the  nerve fiber regeneration after SCI.

Objective To investigate the expressions of urotensin Ⅱ(UⅡ) and tissue transglutaminase (tTG) in rat renal fibrosis tissue and their significances,and provide experimental basis for further study on the mechanism of UⅡ-indced fibrosis. Methods The male Wistar rats were randomly divided into sham operation group (n=8) and model group (n=8).The models were established by unilateral ureteral obstruction (UUO) method.The rats were sacrificed at day 14.The  histological changes in renal tubular interstitium were observed by HE and Masson staining,and the mRNA and protein levels of UⅡ,tTG and fibronectin (FN) were detected by RT-PCR and immunohistochemistry. Results  The Masson and HE staining results showed  interstitial widening and obvious proliferation of collagen fibers in model group.The immunohistochemistry results showed weak signals of UⅡ,tTG and FN in renal tubular cells and tubulointerstitium,and very rare positive signals except UⅡin the glomeruli in sham operation group.Compared with sham opation group,the expressions of UⅡ,tTG and FN in model group were significantly increased (P<0.01) and the level of UⅡ protein had positive correlation with the level of FN protein（r=0.724,P<0.05）. The levels of UⅡ mRNA and protein had positive correlation with the levels oftTG mRNA（r=0.647,P<0.05） and protein（r=0.787,P<0.01）.Conclusion Both UⅡand tTG may play an important  role in the pathogensis of renal tubulointerstitial fibrosis,andUⅡmay promote the development of fibrosis partially via activation of tTG.

Objective To observe the  effects of adequate selenium (Se) and vitamin E (VE) on  myocardial fibrosis and remodelling and  explore theeffects of Se and VE in the repair process after myocardial injury in Keshan disease  area. Methods Thirty Wistar rats were randomly divided into control group (fed on mixed grain),Keshan disease area diet group (EG,fed on Keshan disease area diet continuously),Keshan disease area diet plus Se group (EG+Se),Keshan disease area diet plus VE group (EG+VE) and Keshan disease area diet plus Se and VE group (EG+Se+VE). All rats were sacrificed after 21 d. The activitiy of glutathione peroxidase(GSH-Px) was detected by colorimetry;the expression of malondialdehyde(MDA) in the serum of rats was detected by TBA. The deposition of extracelluar matrix in different groups was detected by mass on trichrome staining. The expressions of TGF-β and CTGF in myocardial cells of rats were detectedby immunohistochemical staining and Western blotting method. Results In the myocardial fibsis rats fed on Kashan disease area diet,the serum GSH-Px activity was decreased,the level of serum MDA was increased,myocardial fabric accumulation and  the expression levels of TGF-β and CTGF were up-regulated.Compared with EG group, the serum GSH-Px activities wre increased (P<0.01 and P<0.05), the MDA contents were decreased(P<0.05),the cardiac extracellular matrix deposition and the expressions of TGF-β and CTGF in rat myocardium were decreased  (P<0.01) in EG+Se group and EG+Se+VE group. Conclusion Low Se and VE play a continuing role in the repair process after myocardial injury. The adequate supplementation of Se and VE isable to reverse the myocardial fibrosis and remodelling.

Abstract:Objective To  study the potential for multiple differentiation of human umbilical cord mesenchymal stem cells (UCMSCs) and discuss the repair effects of UCMSCs in treatment of hypoxic-ischemic brain damage(HIBD) in rats. Methods The UCMSCs were isolated and cultured by using tissue digestion and adherent culture;the expressions of mesenchymal stem cells(MSCs) specific markers of  the P4 and P10 UCMSCs were detected by  flow cytometry,and the aberration condition was judged by chromosomal tests;the potential of multiple differentiation was detected by neurogenic,adipogenic and osteogenic lineages. The active avoidance response rate (AARR) and none avoidance response rate (NARR) were detected by shuttle box test in  normal control,abdominal transplantation UCMSCs and HIBD groups. Results The flow cytometry results showed strong expressions of CD29,CD44 and CD105,and very low expressions of CD34 and CD45 in these.The  immunofluorescence and cell staining results showed that the UCMSCs could be induced into neural cells,adipocytes and osteoblasts;even after high passages.The UCMSCs could still be  differentiated into multiple cells and chromosome aberration did not occur. The shuttle box test results suggested that the AARR in UCMSCs transplantation group was high  than that in HIBD  group on the 3th,4th and 5th days (P<0.05). Conclusion The UCMSCs are easy to be confirmed and purified,and they have multiple differentiation potential,which are still stable with high passages.

Abstract:Objective To observe  the inhibitory  effects of curcumin(Cur) associated with cytokine-induced killer (CIK) cells on  proliferation of ovarian carcinoma SKOV-3 cells in vitro,and  discuss whether they have synergetic anti-tumor effects ifthey are used together and the possible mechanism. Methods The CIK cells were induced from umbilicus cord blood,and the SKOV-3  cells were randomly divided into Cur group,CIK cells group and Cur associated with CIK cells group.The inhibitory rates    of proliferation in  each group  were detected by  MTT method. The mRNA levels  of Fas,Fas associated death domain protein(FADD) and  Caspase-3 in each group  were measured by RT-PCR. Results Compared with  Cur  and  CIK cells groups,the  inhibitory rate of proliferation of  SKOV-3 cells in Cur associated with CKI cells group was increased,and the  inhibitory rate  was increased with the  time prolongation and dose increasing. The inhibitory rate of SKOV-3 cells approached 76.2% when the concentration of effector to taet ratio was 12.5∶1 and  20 μmol·L-1 Cur was used for 〖JP2〗48 h.The mRNA 〖JP3〗levels ofFas and Caspase-3 〖JP〗in SKOV-3 cells were  increased in Cur associated with CIK cells  group compared with  Cur and  CIK cells groups.The mRNA levels of FADD didn’t have significant changes  in various groups.Conclusion The effect of anti-tumor can be enhanced in a synergetic pattern when Cur and CIK cells are used together. Its mechanism may be related with the promoting effects on the mRNA levels of Fas and Caspase-3 in SKOV-3 cells when they are used together.

Abstract:Objective To construct three truncated SCIRR 10 protein mammalian cells systems,and discuss the protein function sites of SCIRR 10. Methods Three truncated SCIRR 10 genes were amplified by PCR,and three kinds of recombinant eukaryotic expression vectors were constructed as follows:pcDNA3.1/Myc-His-SCIRR 10(1-145aa)，pcDNA3.1/ Myc-His- SCIRR 10(1-90aa) and  pcDNA3.1/Myc-His-SCIRR 10(1-70aa). Then they were transiently transfercted intoCOS-7 cells at the same time. After 48 h,the recombinant proteins were detected by Western blotting. Results The molecular weights of  three truncated SCIRR 10 proteins  were    18 800,12 800 and  10 700.Three SCIRR 10 truncated proteins were  successfully expressed in COS-7 cells.β-tubulin protein had no change. Conclusion The three truncated SCIRR 10 protein mammalian cell systems are constructed successfully,and the proteins are obtained by this system.

Abstract:Objective To observe the inhibitory effect of small interfering RNA (siRNA) on the expression    of colorectal cancer related gene PTEN and the influence in the proliferation of colon cancer cells  in vitro.Methods The recombinant plasmids which contained the siRNA of the PTEN gene in the green fluorescence eukaryotic vectors (pGPU6/GFP/Neo-PTEN-1,pGPU6/GFP/Neo-PTEN-2,pGPU6/GFP/Neo-PTEN-3 and pGPU6/GFP/Neo-PTEN-4) were constructed and then they were transfected into colon cancer cells by using Lipofectamine 2 000.The negative control (p5) group and parent cells(p6)group were set up at the same time. The expression of PTEN gene mRNA was detected by real-time PCR. The proliferation of colon cancer cells was detected byMTT. Results Counted by fluorescence microscopy,the transfection rate of cells was 58%. The inhibitory rates of PTEN mRNA in the pGPU6/GFP/ Neo-PTEN-1,pGPU6/GFP/ Neo-PTEN-2,pGPU6/GFP/Neo-PTEN-3 and pGPU6/GFP/Neo-PTEN-4 were 48.3%,76.5%,29.4%, and 61.2%,there were significant differencs compared with p6 group(P<0.05).The proliferation abilities of colon cells were significantly inhibited. Conclusion PTEN gene may play a role in the development of colorectal cancer.

Abstract:Objective To detect the cell uptake and intracellular distribution of silica nanoparticles in HepG2 cells after treated with silica nanoparticles and the cytotoxicity of HepG2 cells caused by the particles and discuss the relationship between intracellular distribution of nanoparticles and cytotoxicity.
Methods The size,shape and dispersibility of silica nanoparticles were detected by transmission electron microscope (TEM) and dynamic light scattering method. The cell uptake and intracellular distribution of silica nanoparticles were observed by fluorescence microscope and TEM. The HepG2 cells were exposed to different concentrations (0,25,50,100 and 200 mg·L-1) of silica nanoparticles for 24 h, the cell viability was reflected by MTT assay and the changes of mitochondrial membrane potential (MMP) were measured by flow cytometry. Results The TEM results  showed that silica nanoparticles were spherical and well dispersed. The dynamic light scattering method results manifested that the size of silica nanoparticles in serum-free DMEM was about 94 nm and well dispersed. Silica nanoparticles could enter into the cells after HepG2 cells were treated with the particles for 3 h. After 24 h treatment,silica nanoparticles were found to disperse in cytoplasm with single particle or cluster form. The particles coulddeposit in some organelles such as mitochondria. After the HepG2 cells were exposed to different concentrations of silica nanoparticles for 24 h,the cell viabiliyand MMP in treated groups were decreased significantly (P＜0.05) in a dose-dependent manner. Conclusion The silica nanoparticles can damage the organelles which is one of the mechanisms of cytotoxicity induced by silica nanoparticles.

Abstract:Objective To study the effects of different protosilencers on gene silencing and the  reasons of differences of gene silencing. Methods HML-I silencer was replaced by different protosilencers. The expression level of reporter gene and the structure of chromosome were compared with or without HML-E. Results After deleting HML-E silencer and replacing HML-I with RAP1p,ORC and Abflp respectively,the cells grew similarly inSC-ura and SC+FOA plate. When HML-E exited,the cells grew similarly on SC-ura plate after replacing HML-I with RAP1p,ORC and ABF1 respectively. The cells were unable to grow on FOA plate when HML-I was replaced with Raplp,However,the cells grew on FOA plate when HML-I was replaced with ORC or ABF1.The micrococcal nuclease digestion data showed that the cells which HML-I was replaced with ORC or ABF1presented similar specific bands to which HML-I was replaced by HMR-E. Conclusion Protosilencers can’t silence gene in activated and basal expression independently,but it can do it with the help of silencer protosilencer. The differences in chromatin structure might be the reason for the difference of gene silencing.

Abstract:Objective To investigate the effect of arsenic trioxide (ATO) on cellcycle progression of imatinib(IM)-resistant chronic myeloid leukemia (CML) cellline KBM5R with T315I point mutation,and provide theoretical foundation for overcoming resistance of CML patients to IM. Methods The wild-type cell line KBM5was selected as negative control of KBM5R with T315I point mutation，and the cells were divided into four groups according to ATO treatment：control group of KBM5R cells,ATO group of KBM5R cells,control group of KBM5 cells and ATO group of KBM5 cells. The proliferation of KBM5 and KBM5R cells after treated with IM or ATO were detected by MTT. The changes of cell cycles of KBM5 and KBM5R cells were quantified by flow cytometry. The expressions of P21 and P27 proteins in KBM5 and KBM5R cells after treated with ATO were determined by Western blotting. Results The IC50 of KBM5R cells treated with IM was higher than that of wild-type cell line KBM5 (P<0.01). The inhibitory rates of proliferation of KBM5 and KBM5R cells after treated with 0.5,1.0,2.0,4.0 and 8.0 μmol·L^-1 of ATO for 24,48,72 and 96 h were higher than that of control group,which were increased according to the increase of concentration or  the treatment time of  ATO （P<0.05）. The inhibitory effect of KBM5R cell oliferation was stronger than that of KBM5 at the same drug concentration and time （P<0.05）. The cell cycle detection of KBM5 and KBM5R cells after treated with 2.0,4.0 and 8.0 μmol·L^-1 ATO for 48 h showed that the G2/M arrest rate was increased according to the increase of concentration of ATO,and the G2/M arrest rate of KBM5R had no signficant difference compared with KBM5 at the same drug concentration. The expressions of P21 and P27 proteins in KBM5 and KBM5R cells after treated with 2.0,4.0 and 8.0 μmol·L^-1 ATO for 48 h were significantly increased in a concentration-depenent manner. Conclusion ATO can arrest cell cycle of KBM5R cell line in G2/M phase through up-regulation of P21 and P27 protein levels,and it is one of thereasons that ATO can inhibit the proliferation of KBM5R cells with T315I point mutation.

Abstract:Objective To investigate the reversal effect of ganoderma lucidum polysaccharides (GLPS) on drug resistance in ovariancancer cells SKOV-3/DDP  and clarifyits drug-resistance  mechanism of GLPS through  improving the resistance genes of ovarian cancer. Methods The ovarian cancer cells SKOV-3/DDP were treated with  different concentration gradients of GLPS (4 ,40 and 400 mg·L^-1) and cisplatin (1,10 and 100 mg·L^-1), the inhibitory rates were detected by MTT method  per 12 h (total test 12-72 h),the experimental cells were divided into control group,GLPS group and cisplatin groupwhen the best inhibitory concentration was gotten. The expressions of  related resistantce genes GSTM1,GSTT1 and GSTP1 were detected by  real-time PCR. The expression differences of GSTM1-related   resistance protein were detected by Western blotting method. Results The inhibitory rates of SKOV-3/DDP cells were higher after treated with  40 and 400 mg·L^-1  GLPS for 12-72 h,and the inhibitory rate was increased with the time prologation and the increase of concentration (P<0.05),and the best inhibitory concentration was 40  mg·L^-1. 10  and 100  mg·L^-1  cisplatin didn’t have effect on the inhibitory rate of SKOV-3/DDP  cells(P>0.05). The expressons of GSTM1,GSTT1 and GSTP1 of OV-3/DDP cells were decreased after treated with 40 mg·L^-1  GLPS  and 10 mg·L^-1  cisplatin for  36 h(P<0.05). The expression of GSTM1 protein was  decreased the most. Conclusion GLPS can inhibit the expressions of drug resistance genes GSTM1,GSTT1 and GSTP1,and improve the resistance effect of tumor cells and enhance their sensitivityies to chemotherapeutic drugs.

Abstract:Objective To investigate the effect of siRNA interfering glucose regulated protein78 (GRP 78) on cisplatin-resistance of human ovarian cancer SKOV3/
DDP cells,and clarify the biomechanism of the effect of GRP78 gene silencing on the cisplatin-resistance of human ovarian cancer SKOV3/DDP cells. Methods The pSilencerTM3.0-H1-GRP78 siRNA plasmid was constructed,then it was transfected into cancer cells by Lipofectamine 2 000. The expressions of GRP78 mRNA and protein were measured by RT-PCR and Western blotting. The expressions of caspase-4 protein and caspase-3 protein were detected by Western blotting. The  apoptotic rate of cells was measured by flow cytometry. Results Compared with control group,the GRP78 gene and protein expression levels  were markedly down-regulated following GRP78 siRNA plasmid transfection(P<0.05);Compared with untransfecting GRP78 siRNA plasmid group,the protein expressions of caspase-4 and caspase-3 in SKOV3/DDP cells in GRP78 siRNA plasmid transfection group induced by cisplatin treatment were sinificantly increased(P<0.05),and the apoptotic rate was also increased(P<0.05). Conclusion The suppression of GRP78 gene expression can increase the  apoptotic rate  of SKOV3/DDP cells through up-regulating the expressions of caspase-4 and caspase-3,so te suppression of GRP78 gene expression can decrease the drug resistance of SKOV3/DDP cells to cisplatin.

Abstract:Objective To study the anti-tumor activity and  effect on immune function of extract from  Bark of Juglans  Mandshurica Maxim （HYT）in orthotopic transplantation of gastric cancer mice and illustrate its underlying mechanism and  provide theoretical basis for  developing traditional low-toxicity and high-performance anti-tumor Chinese medicine and herbs in clinic.  Methods The orthotopic transplantation of gastric cancer mouse models were established with HYT.The mice were divided into high dose of HYT group,lower dose of HYT group,sham operation group,negative control group and positive control group（5-FU）. The inhibitory rate,thymus index and spleen index were observed. ELISA method and flow cytometry were used to detect the IL-4,IL-12,TNF-α levels and T-lymphocyte subsets. Results The difference of the inhibitory rates between high dose of HYT group and positive control group（5-FU）has no sinificant difference（P>0.05）；compared with negative control group,the thymus index and spleen index were increased（P<0.05），the IL-12 level was increased（P<0.05），the IL-4 level was decreased（P<0.05），theTNF-α level was also increased（P<0.05），the   CD4+/ CD8+ ratio of  T lymphocyte subgroup was increased（P<0.05） in high dose of HYT group. Conclusion HYT can enhance immune function and inhibit tumor growth in vivo， and it can be used as  low toxicity and high-performance anti-tumor Chinese medicine and herbs.

Abstract:Objective To explore the inhibitory effects   of  P16 functional oligopeptide on proliferation of mouse C6 glioma cells and human U251 glioma cells cul
tivated in vitro. Methods The C6 cells and U251 cells were treated with  different concentrations of P16 functional oligopeptide (12.5,25.0,50.0 and 100.0 mg·L^-1) for 24,48 and 72 h,the negative control group(without P16 functional oligopeptide) was set up at the same time.The inhibitory rates of proliferation of C6 cells and U251 cells were detected with MTT. Results 12.5,25.0,50.0 and 100.0 mg·L^-1  P16 functional oligopeptide inhibited the proliferation of C6 cells and U251 cells. The inhibitory rates of different doses of  P16 functional oligopeptide were higher than that in negative control group(P＜0.01).The inhibitory rate was increased with the increasing of dose andprolongation of time．Conclusion P16 functional oligopeptide can inhibit the growth of C6 cells and U251 cells.

Abstract:Objective To discuss the optimum temperature and optimum pH of Neanthes japonica (Iznka) fibrinolytic enzyme (NJF) and detect the effects of metal cations and protease inhibitors on the proteolytic activities of NJF and confirm the enzymological classification of NJF. Methods The optimum temperature and pH of NJF were measured by using azocasein as a substrate. The enzyme activities of NJF were determined through ten metal cations (Na^+,K⁺,Cu²⁺,Ca²⁺,Zn²⁺,Co²⁺,Fe²⁺,Fe³⁺,Al³⁺,and Hg²⁺) by using azocasein as a substrate. The enzymological classification of NJF was analyzed by nine protease inhibitors (PMSF,EDTA,EGTA,β-mercaptoethanol,benzamidine,indoaceticacid,aprotinin,pepstatin and soybean trypsin inhibitor). Results NJF had wide thermal stability and pH stability. The enzyme activity was stable between 40℃ and 80℃,and in the pHrange of 7-11. The optimum temperature was 40℃ and the optimum pH was 9.0. The enzyme activity was inhibited completely by Hg ²⁺,and inhibited slightly by Cu²⁺. The other metal cations mentioned above had no obvious effects on the enzyme activity. The enzyme activity was completely inhibited by a typical serine protease inhibitor PMSF. Conclusion NJF is a typical serine protease.

Abstract:Objective To observe the effect of hydrogen sulfide (H2S) on the processing of the amyloid precursor protein (APP) and explore the possible cellular signaling mechanisms in PC12 cells.  Methods The PC12 cells were treated with 50,100 and 200  μmol·L^-1 sodium hydrosulfide (NaHS) for 1 and 18 h. Western blotting method was used to detect the protein levels of C99,C83,APP,Akt,pAkt,ERK1/2 and pERK1/2. The level of Aβ42 in cellular culture medium was analyzed by ELISA. The specific PI3-K inhibitor,LY294002 (25 and 50  μmol·L^-1),or the specific MAPK kinase (MEK) inhibitor,PD98059 (25 and 50  μmol·L^-1),were added to the PC12 cells 30 min　prior to 50  μmol·L^-1 NaHS treatment. Then the above tests were repeated. At the same time normal control group was set up. Results Compared with normal control group,the expressions of C99 and Aβ42 were decreased(P<0.05),the expressions of C83 and pAkt and  the phosphorylation of  ERK1/2 were increased(P<0.05)in 50 μmol·L^-1 NaHS group. LY294002 but not PD98059 inhibited the above effects of NaHS. Conclusion H2S makes APP processing change from the non-amyloidogenic pathway to the amyloidogenic pathway,which may involve PI3-K/Akt signaling pathway.

Abstract:Objective To study the anti-tumor effects of ^125I radioactive particles implantation on transplantated tumor model of human breast cancer cells in nude mice and clarify their anti-tumor mechanisms.Methods 120 nude mice transplantated with human breast cancer cells MCF-7 were randomly devided into 3 groups（n=40）:¹²⁵I radioactive particles implanted group,non-radioactive particles implanted group and non-particles implanted group.The articles were implanted into mice according to Pairs system principle. The expressions of Fas mRNA and protein and the activaties of caspase-3 and caspase-8 enzyme weredetected by RT-PCR and Western blotting.The changes of cell cycle were detected by flow cytometry.Results Compared with non-radioactive particles implanted group and non-particles implanted group,the size of cancer tisses in   ^125I radioactive particles implanted group was reduced significantly(P<0.05).The expressions of Fas mRNA,Fas protein,caspase-3 and caspase-8 were  incresed remarkably(P<0.05).The expressions of  caspase-3 and caspase-8 were more than 0.6. The flow cytometry results showed that the number of cells in G0/G1 phase was significatly increased(P<0.05) while the number of cells in S phase was decreased remarkably(P<0.05). Conclusion The implantation of^125I radioactive particles into transplantated tumor model of human breast cancer cells can kill tumor cells,inhibit the  growth cycle of tumor cells and induce the apoptosis of  tumor cells in nude mice.

Abstract:Objective To discuss the inhibitory effect of the polysaccharide from schisandra chinensis on  human thyroid carcinoma SW579 cells and its mechanism.
Methods 48 adult male Wistar rats were divided into negative control group and treatment group. The rats in negative control group were given 0.9%NaCl.The ones in treatment group were given the polysaccharide from schisandra chinensis pured for different doses. The SW579 cells were divided into control group,negative group and treatment group. The cells in control group were cultivated  in RPMI-1640  medium containing 15% calf  serum.The cells in negative group were cultivated in serum from the rats in negative control group;the cells in treatment group were cultivated in medium containing the rat serum in treatment group including low,medium and high dose drugs(100,200 and 400 mg·kg^-1). The apoptosis of SW579 cells was measured by acridine orange under fluorescence microscope and flow cytometry. The proliferation of SW579 cells was detected by  MTT assay. The expression of survivin was determined by Western blotting method.Results The flow cytometry results  showed that  the apoptotic rates in control group and  negative control group were 4.23% and 4.10%;theapoptotic rates in low,medium and high dose treatment groups were 8.63%，35.63%,and 38.32%,respectively;there were significant differences between treatment groups and control group( P<0.01)The MTT results showed that the inhibitory rate of cell growth in control group was (0.12±0.06)%.The inhibitory rates i low,medium and high dose treatment groups were （27.51±1.62）%，（34.69±0.79）%,and （45.83±1.33）%,respectively;there were significant differences between treatment groups and negative control group(P<0.01).The expressions of survivin in low,medium and high dose treatment groups were lower than those in control group and negative congrol group(P<0.01).Conclusion Polysaccharide from schisandra chinensis can inhibit the survivin expressionthyroid carcinoma  SW579 cells and induce apoptosis of SW579 cells and inhibit cell proliferation

Abstract:Objective To set up H9C2 myocardial cell hyertrophy model with angiotensin Ⅱ(Ang Ⅱ) and  observe the effect of CLC-2 on the hypertrophy process of H9C2 myocardial cells and discuss its mechanism. Methods The H9C2 cells were divided into control group,AngⅡ group,candesartan group,and candesartan plus AngⅡ (candesartan + AngⅡ) group. The changes of H9C2 cell volume in various groups were detected by flow cytometry. The changes of CLC-2 mRNA and protein expressions were detected by RT-PCR and Western blotting methods. Results Compared with control group,the percent of the cells with large volume and the expressions of CLC-2 mRNA and protein in AngⅡ group were increased significantly(P<0.01). Compared with AngⅡ group, the percent of the  cells with large volume and the expression levels  of CLC-2 mRNA and protein in candesartan+AngⅡ group were decreased(P<0.01).
Conclusion AngⅡ can induce hypertrophy of H9C2 myocardial cells,and the mechanism may be related with that CLC-2 can affect the concentration of intracellular chloride.

Abstract:Objective To study the inhibitory effect of secoisolariciresinol diglusoside (SDG) on hyperplasia of prostate induced by testosterone in rats and  clarify itsmechanism. Methods Fifty male Wistar rats were randomly divided into control group,model groups,finasteride (1.0 mg·kg^-1) group and  SDG (1.2 and 0.4g·kg^-1) groups. The levels of NO,SOD,CAT,  TNF-α,bFGF and IL-8 in prostate tissues were detected by ELISA method. Results Compared with  control group,the levels of NO,SOD and CAT in prostate tissues of rats  in model group were decreased(P<0.01),and the levels of TNF-α,bFGF and IL-8  in model group were increased(P<0.01).Compared with  model group,the levels of SOD,CAT and NO  in SDG 1.2 g·kg^-1  group were increased(P<0.01),and the levels of TNF-α,bFGF and IL-8  in  SDG 1.2 g·kg^-1 group were decreased(P<0.01). Conclusion The mechanism of inhibitory effects of SDG on  hyperplasia of prostate induced by testosterone may be concerned with enhancing the activity of antioxidase,clearing away free radical and decreasing the levels of TNF-α,bFGF and IL-8 in rats．

Abstract:Objective To investigate the conformity between the HER2 protein examined by immunohistochemistry (IHC) and the HER2 gene detected with fluorescence in situ hybridization (FISH) and  evaluate the clinical significance.Methods  IHC and FISH were used to detect the expression of HER2 protein and amplification of HER2 gene,respectively,in paraffin-embedded tissues from 77 patients with breast cancer,the conformity beteween the results obtainde by the IHC and FISHwas studided. Results Among 77 cases of paraffin-embedded specimens of breastcancer patients ,37 cases (48.05%) were positive by IHC,while 40 cases were negative (51.95%);HER2 gene amplification was found in 32 patients (41.56%) by FISH,gene non-amplification in 45 cases (58.44%). In 37 patients whose HER2 proteins were positive,HER2 gene amplification was found in 30cases,resulted in a coincidence of 81.08% (30/37). In 40 patients whose HER2 proteins were negative,HER2 gene non-amplification was found in 38 cases ,resulted in a coincidence of 95% (38/40).There was  no significant difference between the two methods( χ2=1.78，P=0.1824).The kappa test result showed that κ=0.7647，there was good agreement between the two methods.Conclusion There were a high coincidence between the HER-2 gene examined by FISH and the HER2 protein detected with IHC.

Abstract:Objective To explore the evidence-based analysis value of cytokeratin 19 (CK19) and epithelial cell adhesion molecule (EpCAM) expressions in gastric cancer. Methods The expressions of CK19 and EpCAM in gastric mucosa (including 31 cases of gastric cancer,7 cases of benign lesions and 16 cases of normal tissues) were detected by microarray-immunohistochemical technique,which were assessed in diagnositic value by evidence-based methods. Results The expressions of CK19 and EpCAM in the benign lesions and gastric cancer tissues were significantly higher than that in the normal tissues (P<0.05). EpCAM had significant positive relevance with infiltration degree(rs=0.514,P<0.05),TNM(rs=0.581,P<0.05),and lymph node metastasis（b=1.376,P<0.05）.The validity,reliability and profit of parallel experiment were higher than those in separate experiment and series experiment of CK19 and EpCAM. Conclusion CK19 and EpCAM are related with the occurrence and development of gastric cancer. The parallel experiment has much more value in evidence-based diagnosis.

Abstract:Objective To study the allelic and genotypic frequence distribution of RETN -420C/G single nucleotide polymorphisms (SNPs) and its relationship with type 2 diabetes mellitus (T2DM) complicated with macroangiopathy in Han population of northeast China.  Methods The genotypes and their frequencies of a total of 180 cases,including 60 cases of T2DM complicated with macroangiopathy,60 cases of simple T2DM and 60 cases of normal control(ND),were measured by PCR-RFLP. Results The allelic frequencies and simple CC/GC+GG genotype inT2DM complicated with macroangiopathy group were significant different compared with  ND group(P<0.01). The allelic frequencies and CC/GC+GG genotype in T2DM comlicated with macroangiopathy  group were significant different compared with T2DM group(P<0.05). The GC+GG genotype in T2DM complicated with macroangiopathy group was significantly higher than that in T2DM group(P<0.05). Conclusion The RETN-420C/G polymorphism has a correlation with the occurrence of T2DM complicated with macroangiopathy in Han population of northeast China.

Abstract:Objective To discuss the expressions of SLeX and CD24 in laryngeal squamaous cell carcinoma(LSCC) tissues and clarify their roles in the occurrence and development in of LSCC. Methods The expressions of SLeX and CD24 in 42 cases of LSCC tissues,24 cases of adjacent tissues,and 20 cases of normal mucosa tisses  were detected by immunohistochemical method . Results The positive expression rates of SLeX in laryngeal cancer tissues group,adjacent tissues group and normal tissues group were 71.4%，37.5%,and 30.0%,respectively. The positive expression rates of CD24 in laryngeal cancer tissues group,adjacent tissues group and normal tissues group were 64.3%，33.3%,and 25.0%,respectively. The strong positive expression rates of SLeX in laryngeal cancer tissues group,adjacent tissues group and normal tissues group were 50.0%，8.3%,and 5.0%， respectively. The strong positive expression rates of CD24 in laryngeal cancer tissues group,adjacent tissues group and normal tissues group were 35.7%，4.2%,and 0.0%,respectively. The positive expression rates of SLeX and CD24 had  significant  differences among laryngeal cancer tissues,  adjacent tissues and normal tissues (P<0.01). There was no significant difference between adjacent tissues and normal tissues (P>0.05). The strong positive expressions of SLeX and CD24 had significant correlation with the metastases of the cervical lymph nodes,the clinic stages and histopathological classification. The expression level of SLeX had positive correlation with CD24 in LSCC tissue (r=0.695,P<0.05).Conclusion The expressions of SLeX and CD24 are related to the occurrence and development of LSCC. The detection of the expressions of SLeX and CD24 has clinical significance on  biotherapy and prognosis of LSCC.

Abstract:Objective To investigate the incidence of intraoperative awareness using bispectral index (BIS) monitoring and evaluate the preventive effect of BIS on intraoperative awareness under total intravenous anesthesia. Methods 300 patients undergoing total intravenous anesthesia with BIS monitoring were randomly divided into two groups:group A and group B (n=150). In group A, the depth of anesthesia was regulated lower than 60 according to BIS. While in group B,the BIS monitor was covered and the depth of anesthesia was modulated according to the experience of the anesthesiologist. The BIS values were recorded at the time of anesthesia induction,beginning of operation,anesthesia withdrawal,end of operation,recovery of anesthesia and extubation. Results The basic BIS and BIS values at the time of anesthesia induction,awake and extubation had no significant differences between two groups (P>0.05). The BIS values at the time of beginning of operation,anesthesia maintenance and anesthesia withdrawl in group B were significantly higher that those in group A (P<0.05). The patients with awareness and suspected of awareness were not found in group A. The patients with awareness were not found in group B but there were two patientssuspected of awareness. The incidence of patients suspected of awareness had significant difference between two groups (P<0.05). Conclusion BIS monitoring during total intraveous anesthesia could maintain the appropriate depth of anesthesia and prevent intraoperative awareness.

Abstract:Objective To investigate the expression of  macrophage inflammatory protein(MIP)-1α in inflammed pulp tissus,and elucidate its potential role in the development of pulpitis. Methods 4 cases of normal pulp tissues and 4 cases of inflammed pulp tissues were selected. The histomorphologic changes  of normal pulp tissues and 4 inflammed pulp tissues were examined by HE staining. The mRNA expressions of MIP-1α in normal pulp tissues and 4 inflammed pulp tissues were detected by using the reverse transcriptase polymerase chain reaction (RT-PCR). Results
The fibroblasts,collagens fibers,capillaries and histiocytes were observed in the normal pulp tissues by staining. The vasodilatation and hyperemia,lymphocytes,plasma cells,macrophage and neutrophils infiltration,vascular proliferation and collagenous fibers were observed in the inflammed pulp tissues. The expression rate of MIP-1α mRNA in normal pulp tissues was 0,and the expression rate of MIP-1α mRNA in inflammed pulp tissues was 100%.The relative expression amounts of each inflammed example were 1.07,1.11,1.21,and 0.96. There was significant difference of MIP-1α mRNA expression between two groups with the analysis of exact probabilities（P<0.01）. Conclusion There is not expression of MIP-1α in normal pulp tissues，while there is expression of MIP-1α  in inflammed pulp tissues.MIP-1α may play an important role in the development of pulpitis.

Abstract:Objective To study the changes of retentive force during the circulation of the acetal resin clasp dislodging and inserting and discuss the proper depth of the abutment undercut and cross-section diameter of the acetal resin clasp. Methods The instron force measuring instrument was used to record the variation of the retentive force of acetal resin and Co-Cr alloy casting akers clasp when they were dislodging 2 880 times from abutment undercuts of different depths (0.50,0.75,and 1.00 mm) and different diameters of the acetal resin clasp (1.20 and 2.00 mm) on bicuspid. Results The variations of the retention in the measurement were aceording to  different undercut depths and different diameters. The value of acetal resin clasp’s retentive force became largerwith the increasing of the diameter of cross-section of the clasp and the  depth of abutment undercut（P<0.05）.  Conclusion The  acetal resin clasp of 2.00 mm diameter and the abutment undercut with 0.75 mm are suitable for bicuspid.

Abstract:Objective To observe the losing ratio of the bond orthodontic brackets in fluorotic teeth by using different etching methods and find a way to decrease the losing ratio of the bond orthodontic brackets in fluorotic teeth. Methods 226 midrange fluorotic teeth from 14 patients were chosen. The teech were divided into 28 sections according to the direction.Then the 28 sections were randomly divided into four groups: etching 1 min group(n=56),etching 2 min group（n=57）,etching 3 min group（n=57） and etching twice group (n=56). The losing ratios of brackets and adhesive remnant index (ARI)were observed after 4,8 and 12 weeks. Results The losing ratios of brackets in etching 2 min group and etching twice group were lower than those in etching 1 min group and etching 3 min group(P<0.05). The ARI in etching 2 min group and etching twice group were higher than those in the other two groups(P<0.05). The losing ratio of brackets in etching twice group was lower than that in   etching 2 min group，but there was no significant difference between two groups(P>0.05). The ARI in etching twice group was higher than that in etching 2 min group,but there was no significant difference between two groups(P>0.05). The losing ratio of brackets on bicuspid teeth were higher thanthose on front teeth in each group. The losing ratio of brackets on bicuspid teeth in etching twice group was lower than those in the other groups(P<0.05). Conclusion Etching  2 min can reduce the losing ratio of the bond orthodontic brackets in fluorotic teeth. The losing ratio of the bond orthodontic brackets in etching twice group is lower than that in etching once group,ecially on bicuspid teeth.

Abstract:Objective To investigate the changes of nickel and chromium contents in gingival tissue and gingival crevicular fluid (GCF) after porcelain-fused-to-nichrome crown restoration and provide a reference for the choosing of materials.Methods The patients who needed partial gingival excision with hyperplasia gingival after porcelain-fused-to-nichrome crown restoration were selected as test group. The patients without any restoration in mouth whose wisdom teeth needed to be removed were selected as control group. Whatman 3# strip filter paper was usedto collect GCF,and excised gingival was collected. The content changes of nickel and chromium in gingival tissue and GCF were detected by inductively coupled plasma mass spectrometry (ICP-MS). Results The contents of nickel and chromium in gingival tissue and GCF in test group were higher than those in control group,andthe content of chromium in GCF in test group was significantly higher than that in control group (P<0.05),but there was no significant difference in nickel content between test and control groups (P>0.05). Conclusion The contents of nickel and chromium in gingival tissue and GCF have an increasing tendency after porcelain-fused-to-nichrome crown restoration,and they have an effect on gingival tissue.

Abstract:Objective To compare the  curative effects of mastoscopy and conventional surgery in the treatment of breast diseases and study the advantages and disadvantages of the two methods. Methods 20 patients with breast cancer,benign tumor,polyacrylamide hydrogel injection or gynaecomastia underwent mastoscopy-assisted surgery were collected as mastoscopy group and 100 patients  underwent conventional surgery in the same period  were collected as control group. The aesthetic result，the operation time,the treatment time and  the complications were assessed in two groups. Results The consmetic outcome achieved in 95% patients in mastoscopy  group while in 34% patients in control group(P<0.01). There were no differences  in the complications of  hematoma,seroma,infection and  recurrence between  two groups(P>0.05). The postoperative pain and the incidence of injury of mastoscopy were shorter than that of conventional surgery.Mastoscope-assisted breast augmentation could reduce the pain,bleeding and tissue injury (P<0.05).The surgical incision in mastoscopy group was smaller and the treatment time was shorter than  those in control group(P<0.05). Conclusion The surgery assisted by mastocopy can achieve better cosmetic results. It is a good way to perform surgery for the patients with all kinds of breast diseases.

Abstract:Objective To observe  the incidence of pain after treatment of acute periapical periodontitis using negative pressure following opening drainage. Methods 142 patients with acute periapical periodontitis were randomly divided into experimental group(72 teeth) and control group(70 teeth).The patients in experimental group were treated with negative pressure drainage;the patients in control group were treated with conventional opening drainage. The incidence and degree of  pain after root canal preparation and the changes of IL-1β,PGE2 and CRP  levels  intwo groups were recorded. Results There was significant  difference of VAS difference value between two groups（P<0.05）.The changes of IL-1β,  PGE2  and CRP levels  in ex
perimental group were larger than those in control group（P<0.05）.The effective rate of treatment in experimental group was higher than that in control group  (P<0.05). Conclusion The negative  pressure drainage can effectively prevent and reduce the pain during root canal therapy of acute periapical periodontitis.

Abstract:Objective To investigate the metastatic pattern of lymph node around rectum in theextensive radical mastectomy   of rectal carcinoma protecting  pelvic splanchnic nerve, and explore  the significance of lateral lymphnode  clearance in rectal carcinoma. Methods 114 rectal carcinoma patients were treate with  extensive radical mastectomy for rectal carcinoma  protecting  pelvic splanchnic nerve. The lymph nodes were cleared and observed by  conventional pathological method (hematoxylin and eosin staining), and  the metastatic pattern of lymph nodes around rectum was analyzed. Results Among 114 patients, 46 patients had metastasis in lymph nodes, the total metastatic rate was 40.4%(46/114). The metastatic rates of mesenteric lymph nodes, lymph nodes around inferior mesenteric artery and lateral lymph nodes were 39.5%(45/114), 3.9%(2/51), and 19.6%(11/114), respectively. Total 2 463 lymph nodes were cleared and 209 lymph nodes were  metastasized,the total metastatic extent was 8.5%(209/2 463). The metastatic extent for absorbent gland of mesenteric lymph nodes, lymph nodes around inferior mesenteric artery and lateral lymph nodes  were 14.8%(187/1 264), 1.7%3/175), and 1.9%(19/1 024). The metastatic extent for absorbent gland and metastatic rate  of  mesenteric lymph nodes were higher than  those of lymph nodes around inferior mesenteric artery and lateral lymph nodes（P<0.05）. The metastatic rate and extent of mesenteric lymph nodes were related to the  Duke’s stage and the infiltration  extent（P<0.05）. And the matastatic rate and extent of lymph nodes of lateral lymph nodes were related to the Duke’s stage and differentiation extent（P<0.05）. Conclusion The slective  extensive radical mastectomy for rectal carcinoma is feasible .

Abstract:Objective To discuss the ischemic information with dynamic susceptibility contrast-enhanced MR imaging（DSC-MRI） and artery spin labeling（ASL）technique in the patients with unilateral middle artery stenosis,and confirm the diagnosis value of the ASL perfusion technique in ischemic cerebravascular diseases. Methods 48 patients of transient ischemic attack（TIA）  with unilateral middle cerebral artery (MCA) stenosis or occlusion and 35 healthy volunteers were collected.  The concentration-time curve was got through cerebral blood flow (CBF) map and DSCimages. The difference of the cerebral perfusion in side of the MCA  stenosis was observed by comparing the concentration-time curve between the two methods. Results The rCBF of  ipsilateral MCA stenosis tissues was  （30.4±8.2）mL·100 g^-1·min^-1 and （58.3±11.4） mL·100 g^-1·min^-1  of contralateral tissues detected with  ASL-PWI method ,there was significant difference(P<0.05).The rCBF in  ipsilateral MCA stenosis tissues was （28.7±12.8） mL·100 g^-1·min^-1 and  （54.2±9.5） mL·100 g^-1·min^-1  of contralateral tissues detected with DSC-PWI method(P<0.05).The rCBF of ipsilateral tissue of the same blood vessel detected with ASL andDSC had no significant difference(P>0.05). The rCBF of healthy controls detected with ASL and DSC were （56.8±0.7） mL·100 g^-1·min^-1  and （55.2±9.8） mL·100 g^-1·min^-1 ,there were no significant differences compared with controlateral tissue of MCA stennosis(P>0.05).The apparent diffusion coefficient (ADC) value of ipsilateral MCA stenosis was （522.3±57.2×10^-6）mm²·s^-1 and （756.8±67.6×10^-6）mm²·s^-1  in contralateral tissue detected with DWI method,there was significant differerce(P<0.05). Conclusion ASL can measure the CBF data and  the extent of low perfusion in MCA  stenosis and occlusion;ASL in combination with DWI can indicate the ischemic penumbra in MCA stenosis patients.

Abstract:Objective To isolate the human keratinocyte stem cells(hKSCs) in vitro and evaluate  their biological characteristics. 
Methods The normal skin was taken by circum cision and the hKSCs were digested by enzyme and isolated by rapid adherence screening method. The morphology and ultrastructure of hKSCs were observed by phase contrast microscope and transmission electronic microscope,and the molecular markers were identified with immunocytochemistry and indirect immunofluorescence and the cell cycles were detected by flow cytometry.Results With phase contrast microscope,the rapidly adherent cells were  uniformed small, large nucleus  and epithelial-like. There were positive expressions of  cytokeratin 19,cytokeratin 15,p63 and CD29 by immunocy tochemistry,immunofluorescence  and flow cytometry, while there were negative expressions of vimentin and cytokeratin 10,which were the specific markers of fibroblasts and epidermal terminal differentiation respectively. The result of RT-PCR  was the same to the above. Most cells were in G0/G1 stage  by cell cycle analysis which suggested that hKSCs had slow cell cycling nature.  Conclusion hKSCs can be isolated and cultivated  in vitro successfully and they can be used as cell sources in the further experiments.

Abstract:Objective To establish the method of using the 12th vertebrae for sex determination of adult Chinese and evaluate its effect. Methods The 12th thoracic vertebrae were developed by the clinic abdomen CT images. A total of 25 linear measurements on 7 aspects of the vertebrae were measured and 4 ratios were calculated. The items were selected which had the significant difference to establish the sex determination equation and its  effect was evaluated.  Results Of the total 29 traits,27 were sexually dimorphic(P<0.05),the accuracy was 56.3%-89.2%,8 traits had the accuracies mean or over 80.0%.The trait iVL had the highest accuracy of  89.2%. A function with four variables predicting sex with 90.8% accuracy was derived by using stepwise method of discriminant function analysis:Y=2.98×iBDsm+1.97×PH+3.37×BHp+3.27×sVL/BHa－32.80(mean centroids=－7.69). Conclusion The method of using the selected traits for sex determination of adult Chinese is practicable and it has a relatively high accuracy.

Abstract:Objective To  extract and purify the  rombinant protein LTB-FAP of  fimbria of Actinobacillus actinomy cetecomitans which can be expressed in E.coli. and lay a foundation for its application in the treatment of periodontal diseases.Methods The constructed recombinant plasmid of  pET28a/LTB and pET28a/LTB-FAP  were  transformed into E.coli and the inclusion body of recombinant protein LTB-FAP was expressed. The recombinant protein was washed and dissolved by 2,4,6 and 8 mol·L^-1 urea;and then the dissolved protein was purified by both anion exchange chromatography and gel filtration chromatography;the concentration ofthe purified protein LTB-FAP was measured by thin layer chromatogram scanner.Results The inclusion body of the expressed protein was washed 3 times by 4 mol·L^-1 urea solution and dissloved in 6 mol·L^-1 urea solution,and 60% of the LTB-FAP protein could  be gotten. The purity of the recombinant protein LTB-FAP  purified in this experiment by anion exchange chromatography was about   75%;the puity of recombinant protein  LTB-FAP  was  90% by gel filtration chromatography. Conclusion The anion exchange chromatography and gel filtration chromatography can extract and purify the expressed recombinant protein LTB-FAP which can be used to immunize animal bodys. 

Abstract:Objective To investigate the types of the mental disorders  and the disease characteristics of schizophrenia,mood disorder,hysteria in inpatients and analyze their relevant risk factors. Methods A total of 2 453 clinical records of mental disorders inpatients according to CCMD-II-R were collected in Yantai from 2008 to 2010 by self-designed questionnaire. Results The number of female patients was more than male patients,and the male to female ratio was 0.74∶1 The average age of inpatients was （38.74±15.45） years,the average age of onset was (35.98±16.08) years.The male patients had earlier onset age than female (P<0. 001). The proportions of schizophrenia,mood disorder and hysteria were 45.1%,26.3%, and 7.8%.  The schizophrenia inpatients had first onset among the  three diseases. The hysteria inpatients had the lower education,and were more likely to have disease history and causes,and had less smoking and less drinking  than the schizophrenia inpatients (P<0.05). The mooed disorder inpatients had more people who lived in city or town,they wer more likely to have disease history,drinking history and causes(P<0.05). Conclusion The different mental disorder inpatients have different disease characteristics. More specific easurements of prevention and treatment for decreasing occurrence of this disease are needed.

Abstract:Objective To provide epidemiological data of non-infectious chronic diseases containing hypertension,diabetes mellitus(DM),dyslipidemia,alcoholic fatty liver disease(ALD),non-alcoholic fatty liver disease(NAFLD),metabolic sydrome(MS),overweight and central obesity and determine their risk factors in Dehui city in Jilin Province. Methods A total of 6 043 individuals including 11 neighborhoods and 9 villages were chosen based on cross-section study. A multivariate logistic regression was conducted between non-infectious chronic diseases and those risk factors.Results The prevalences of hypertension,DM,dyslipidemia,ALD,NAFLD,MS,overweight and central obesity were reported as 28.67%，4.82%，51.00%，3.74%，15.5%，21.54%，58.22%, and 36.67%, respectively, and the prevalence of hypertention was higher in males than that in females(P<0.001),and the results were reversed in central obesity,dyslipidemia，MS and NALD(P<0.05).A multivariate logistic regression analysis showed  that the OR were 1.04(95%CI：1.03-1.05) for age,19.42(95%CI：11.62-32.4) forfatty liver,1.15(95%CI：1.06-1.26) for FBG,1.38 (95%CI： 1.19-1.59) for hypercholesterolemia,1.32(95%CI：1.1-1.49) for hypertriglyceridemia,1.42(95%CI：1.3-1.47)for BMI,which were the main risk factors for non-infectious chronic diseases.
Conclusion Non-infectious chronic diseases are very common among adult population in Dehui. As people get older the prevalence of various chronic diseases gradually increases .The occurrence and development  of non-infectious chronic diseases has significant relation with life habit,dietary habit,economic level and education.