Abstract:

Abstract:Objective To investigate the potential role for Hath1 in pathogenesis of  colon adenocarcinoma. Methods The  sections of paraffin-embedded tissues were used to investigate the goblet cell population of normal colon mucosa,mucosa adjacent colon cancer and colon cancer tissues obtained  from 48 patients with non-mucinous colon adenocarcinoma. ThemRNA and protein  expressions of hath1 and Muc2   in these samples were tested by quantitative real-time reverse transcription -PCR,immunohistochemistry andWestern blotting. The Hath1 gene was transfected into  colon cancer cells HT29,then three clones were selected randomly to test the expressions of mRNA and protein of Hath1 and Muc2 by quantitative real-time reverse transcription-PCR and Western blotting.The protein expressions of cyclin D1 and p27 were also tested by Western blotting. Moreover,the proliferativeability of HT29 cells transfected with Hath1 was observed by means of colony formation assay and xenograft. Results There were not any goblet cells to be found in colon cancr. The expressions of mRNA and protein of Hath1 and Muc2 were significantly down-regulated in colon cancer（P<0.05）. Hath1 could decrease the expression of cyclin D1 protein（P<0.05）,increase the expressions of p27 protein（P<0.05） and Muc2 protein（P<0.01） in HT29 cells,inhibit these cells to form colonies（P<0.01） and decrease the size of xenografts（P<0.01）. Conclusion Down-regulation of Hath1 may concern with non-mucinous colon adenocarcinoma,Hath1 may be an anti-oncogene in pathogenesis of non-mucinous colon adenocarcinoma.

Key words: Hath1;colon adenocarcinoma;colon carcer cell line;cell cycle regulation