Abstract:

Abstract:Objective To investigate the relationship between multi-drug resistance relative protein-2 (MRP-2) and leukemia multi-drug resistance，and  study its effect on leukemia multi-drug resistance.Methods The expressions of MRP-2 in leukemia cell line K562 and leukemia multi-drug resistance cell line K562/A02 were detected by PT-PCR.Anti- and oligonucleotide of MRP-2 were transfected by lipidosome into K562/A02,intra-cellular doxorubicin (ADM) fluorescence intensity,resistance index,and the expression of MRP-2 mRNA were detected by MTT,flow cytometry,and RT-PCR.Results The expression of MRP-2 in K562/A02 was higher than that of K562(P<0.05).After transfected with anti-oligonucleotide MRP-2,the ADM fluorescence intensity of K562/A02 was increased,and the resistance index was significantly decreased,and the expression of MRP-2 mRNA was   decreased by 67.5% (P<0.05) compared with untransfected cells.Conclusion The high-level expression of MRP-2 results in the reducing of chemical drug concentration,which may be one of the  mechanisms of leukemia multi-drug resistance.

Key words: leukemia;multi-drug resistance;multi-drug resistance relative protein-2