Abstract:

Abstract:Objective To detect the expressions of triggering receptor expressed on myeloid cells-1(TREM-1),TNF-α and IL-8 of foam cells derived from macrophages.Methods Human U937 cell strain was induced by PMA and then was divided into PMA  group,LDL  group and ox-LDL  group.RT-PCR was applied to measure the mRNA expression of TREM-1 in each group and ELISA was used to detect the contents of TNF-α and IL-8  in culture supernatant in each group.Results Compared with PMA group and LDL group,the TREM-1 mRNA expression in ox-LDL group was significantly increased(P<0.05);the TNF-α and IL-8 contents in culture supernatant in ox-LDL group were  also significantly increased(P<0.05).Conclusion TNF-α,IL-8,and TREM-1 maybe play an important synergetic effect in the process of atherosclerosis.

Key words: foam cells/macrophages;triggering receptor expressed on myeloid cells-1;TNF-α;IL-8;atherosclerosis