Abstract:

Abstract:Objective
 To approach the spectrum of pathogen of candidal vaginitis and analyze the randomly amplified polymorphism DNA (RAPD) of isolated pathogen,and provide basis for  clinical diagnosis and epidemiological surveillance of candidal vaginitis.Methods The specimens of vaginal discharge of 106 patients suspected with candidal vaginitis were collected.Pathogenic fungal were isolated and identified.10 random primers (RAPD 1-10) were used to make RAPD analysis on the isolates.Results ①72 strains of Candida were isolated,among which C.albicans was 56 strains (77.78%),non-Candida albicans Candida (NCAC) species was 16 strains (22.22%) including 6 strains of C. glabrata (8.33%),4 strains of C. tropicalis (5.56%),1 strains of C. krusei (1.39%) and 5 strains of other Candida sp. (6.94%).②RAPD analysis result showed that 2 primers (RAPD2 and RAPD5) could have better clear and stabile specific pattern bandings with evident interspecies genetic variability and intraspecies genetic resemblance.Conclusion  C.albicans is the main pathogen of candidal vaginitis.RAPD2 and RAPD5 are suitable for identification and typing of C.albicans and C.tropicalis.

Key words: Candida;candidal vaginitis;randomly amplified polymorphism DNA