Abstract:

Abstract:Objective
To study the inhibitory effects of cadmium chloride (CdCl2) on SMMC-7721 cells in hepatocarcinoma    transplanted nude mice and influence in mitochondrial enzymes and clarify their mechanisms. Methods The hepatocarcinoma transplanted nude mouse model were established in vivo. The mice in negative control group were administered with sodium chloride,the mice in positive control group were administered with 25 mg.kg^-1 　5-Fu，and the mice in Cdcl2 groups were administered with   0.5,1.0,2.0 mg.kg^-1 1 CdCl2,respectively. The anti-tumor effects of CdCl2,the changes of serum ALT and AST levels, the activities of LDH and ATPase in mitochondria were detected 10 d after administration. Results Compared with positive control group,the inhibitory rate of tumor tended to increase along with the elevating of CdCl2 dose,but there were no statistical significances (P>0.05). The organ indexes in all CdCl2 groups didn’t change greatly,but the kidney organ indexes  in CdCl2 groups were higher than that in positive control group (P<0.05). Compared with  negative control group,the level of ALT and  the activity of LDH in CdCl2 groups were significantly decreased(P<0.05),and the level of AST in 0.5 and 1.0 mg.kg^-1 CdCl2 groups were also  significantly decreased(P<0.05).The activities of Ca ²⁺ -Mg ²⁺ -ATPase in 1.0 and 2.0 mg.kg^-1  CdCl2 groups were significantly increased compared with  negative control group(P<0.05),the activities of Na ⁺ -K⁺-ATPase in 0.5 and 2.0 mg?kg-1 CdCl2 groups were  significantly increased  compared with  positive control group(P<0.05). Conclusion CdCl2  may interfere the energy metabolism of mitochondria and  inhibit the growth of  the transplanted hepatocarcinoma through mitochondria way.

Key words: cadmium chloride;liver neoplasms;lactate dehydrogenase;andenosinetri phosphatase;mice,inbred BALB/C