J4 ›› 2011, Vol. 37 ›› Issue (1): 51-55.
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LIU Ji, JIA Tian-Jun, LIU Xue-Qing, JIA Xiao-Hui, ZHAI Xue-Qiong
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Abstract:
bstract:Objective To obain the C terminal gene encoding human mannose-binding lectin associated serine protein kinase-2(MASP-2),and express human MASP-2 C-terminal fragment and clinical application in E.coli and lay foundation for preparation of monoclonal antibodies and clinical application in relevant diseases. Methods The cDNA of human MASP-2 C-terminal was amplified from total RNA extracted from human fetal liver tissue with RT-PCR,and was cloned into pGEX-6p-2 vector and identified by restrictive digestion analysis and sequencing. Results The cDNA encoding human MASP-2 C-terminal was isolated,linked with pGEX-6p-2 vector and transformed into E.coli XL1-blue. The restriction map analysis and sequencing results were consistent with the sequence of MASP-2 C-terminal cDNA that reported in GenBank. Conclusion The human MASP-2 C-terminal cDNA is successfully cloned and expresses in E.coli.
Key words: mannose-binding lectin associated serine protein kinase-2 C;reverse transcriptase polymerase chain reaction;cloning,molecular;gene expression
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LIU Ji, JIA Tian-Jun, LIU Xue-Qing, JIA Xiao-Hui, ZHAI Xue-Qiong. Cloning and identification of C-terminal gene encoding human mannose-binding lectin associated serine protein kinase-2[J].J4, 2011, 37(1): 51-55.
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