Abstract:

Objective To detect the expressions of  focal adhesion kinase (FAK) and extracellular signal-regulated protein kinase（ERK）in  salivary adenoid cystic carcinoma (SACC) cells SACC-LM and SACC-83 and explore their relationship with occurrence of lung metastasis of SACC. Methods Western blotting was applied to detect the  protein expressions of  FAK and ERK  in SACC-LM and SACC-83 in G1/S phase and the enzyme activities of FAK and ERK,the results were quantified. Results The activities of FAK and ERK in SACC-LM cells were higher than those in SACC-83 cells（P<0.01）,the activity of FAK in SACC-LM cells had positive correlation with the activity of ERK(r=0.62，P<0.05).The Western blotting results showed  that the  protein expression levels of FAK and ERK in SACC-LM cells were higher than those in  SACC-83 cells（P<0.05）. Conclusion FAK and ERK may be involved in the occurrence and development process and metastasis signal conduction passage of SACC,the protein expressions of FAK and ERK concerns with the variation behavior of SACC.

Key words: focal adhesion kinase;extracellular signal-regulated kinase;salivary gland neoplasms;SACC-LM cells;SACC-83 cells;protein expression