Abstract:

To observe the repairing effect of marrow derived mesenchymal stem cells (MSCs) on hepatic fibrosis in rats，and provide a reliable theoretical basis for further clinical application of MSCs.Methods The MSCs of rats were isolated,purified and cultivated in vitro.The model of hepatic fibrosis induced by CCl4 was established.The rats were divided into three groups:normal control group,model group,MSCs transplantation group,6 each group.The MSCs were transplatated into the rats through tail vein in MSCs transplantation group.The repairing effect of MSCs on hepatic fibrosis was observed  and the expression of α-SMA in liver tissue was detected by immunohistochemistry  30 d after transplantation.Results  93.27% MSCs were at G0/G1 phase,and the CD44 surface expression in the MSCs was positive.The hepatic pathological injury changes of rats in MSCs transplantation group were significantly reduced than that in model group  30 d after MSCs injection.The lobular structure and liver sinusoids were normal and hyperplasia of fibrous connective tissue was not obvious.The intensity of α-SMA staining in MSCs transplantation group was lower than that in model group.Conclusion MSCs have  repairment on hepatic fibrosis in rats to a certain extent.

Key words: mesenchymal stem cells;hepatic fibrosis;α-SMA；tissue repair