Abstract:

Abstract:Objective To establish the determination method of total polyphenols in vitis amurensis seed extract.Methods Using gallic acid as reference substance，the content of total polyphenols in Vitis amurensis seed extract was determined by the optimum conditions of Folin-Ciocalteu chromatometry.Results There was a good linearity between gallic acid concentration and absorbance in the range 0.002-0.010 g·L^-1（Y=86.243X+0.007,r=0.9996）；the precision RSD was 0.14%；the stability RSD was 1.77%； the repeatability RSD was 1.44%； the average recovery rate was  102.00%（RSD=1.37%）.Conclusion This method is simple，fast and has high sensitivity and good reproducibility.

Key words: Vitis amurensis seeds；total polyphenols；gallic acid； chromatometry